In Vitro Cell Culture Service

Cell Culture: a Powerful Tool

Creative Biolabs is a leading provider of cell culture services to support the research community. The quality control of our cell culture services is stringent. Cell line authentication, mycoplasma, sterility, viral, viability, and sterility testing are all included. We also offer customized cell culture systems and make sure to meet the high standards of the clients needed. Numerous types of cell culture models are available to study the pathophysiological mechanism.

Our Capabilities

Available cells: Primary human cells, mammalian cells, insect cells, tumor cell cultures, and hybridomas.

We provide custom cell culture services for epithelial cells, fibroblasts, adipocytes, muscle cells, neurons, pancreatic cells, and other tumor cells. We also offer a range of oncogene-free induced pluripotent stem cells (iPS), which include human multipotent stem cells, human adipose mesenchymal stem cells, CD34⁺ cord blood stem cells, and other stem cells. Moreover, culturing of mammalian cells for the production of therapeutic proteins and monoclonal antibodies is also our strength. Our cell culture experts also perform numerous types of downstream assays with the cells.

Custom Cell Culture Services

• Scale-Up
• Billions of adherent cells
• Thousands of liters of suspension cells per month
• Large quantities of cell-derived bioreagents each month
• Large single batches of up to 1,500 vials per bank
• Ready-to-Use Cells
• Providing ready-to-use cells in the preferred format according to the clients' specifications
• Reducing experiment to experiment variability
• Cell Line Engineering
• Stable cell lines or transiently transfect cell development
• Verify protein expression using ELISA, IHC, western blot, and flow cytometry
• Stringent Quality Control
• Cell line authentication by a unique barcode
• Mycoplasma testing: Specific PCR-based methods
• Sterility testing: Test of bacteria and mold
• Viral testing: Viral and pathogen testing by PCR-based methods
• Viability: the viability of all cell banks pre- and post-thaw.
• Cell Banking and Storage

Fig.1 Cell maintenance.¹

Our Featured Cell Culture Services

• PBMC and isolated immune cell subset cultures
• T cell proliferation and expansion
• Macrophage differentiation
• Dendritic cell differentiation
• Short-term spheroid cultures
• Cultures Supporting the study of therapeutics

Applications of Cell Culture

• Used as Experimental model systems in basic sciences
• Studies of the culture conditions for certain cell types
• Studies of cell expansion and differentiation
• Determination of the genetic status of cells
• Cell engineering

What Do We Use Cell Culture for?

• Immuno-pharmacological characterization
• Immuno-toxicological characterization
• Customized cellular development
• Consulting services for immunologically active drugs
• Experienced in the handling of complex biological materials

Other Cell Culture and Assay Services

• 3D cell culture in vitro modeling
• 3D spheroids
• Invasion Assay
• Co-culturing
• Mesenchymal stem cells (MSCs)
• Custom disease model
• Large-scale culture
• Cell morphology
• Cell growth/tumorigenicity
• High-content image analysis
• Image-enabled cell line generation
• Cytotoxicity/proliferation assay

We Also Provide:

• In Vitro and Ex Vivo Model Services
• In vitro testing of immortalized cell lines, primary cell lines, and induced pluripotent cells.
• Ex vivo testing: Giving a picture closer to biological reality, this assay is performed on tissue samples.
• Cell Adhesion Model Assay Services

After cell cultures with known conditions, plasmids, compounds, and other details are fully understood. Cell adhesion assays are performed to look at the adhesion of cells in the presence of extracellular matrix (ECM) proteins (for example: collagen or fibrinogen). Then, non-adherent cells are removed to measure the remaining adherent cells.

Integrated solutions at Creative Biolabs simplify cell culture projects by streamlining access to source cells and accelerating downstream analyses to quickly and cost-effectively provide actionable data for drug and diagnostic development programs. For more detailed information about in vitro cell culture service, please feel free to contact us.

Reference

1. Segeritz.; et al. "Cell culture: Growing cells as model systems in vitro."Basic science methods for clinical researchers. Academic Press, 2017. 151-172.

For Research Use Only | Not For Clinical Use