PharmaOnco™ C57BL/6 Mouse CD3 Pan T Cells, Purified

CAT#: IC-LX048
Product Type: OncoImmune Cells
Cell Type: CD3 Pan T Cells
Species: C57BL/6 Mouse
Short Description
Creative Biolabs offers C57BL/6 Mouse CD3 Pan T Cells, Purified for a variety of stimulation-dependent functional experiments to assess cytokine production and cytotoxicity.
Description
C57BL/6 Mouse CD3 Pan T Cells, Purified are isolated T cells from the CD3-expressing population in the spleen or lymph nodes of C57BL/6 mice. These purified T cells can be used for various experiments and studies in immunology and cell biology research, such as studying T cell activation, proliferation, differentiation, and function. They can also be used in adoptive transfer experiments or in vitro assays to investigate T cell responses to antigens or immunomodulatory molecules.
Species character
The C57BL/6 mouse is the most commonly-used inbred mouse strain and is employed in numerous research areas, including immunology, genetics, diabetes and obesity, neurobiology, cardiovascular biology, and developmental biology. They are routinely used as the background strain for generation of congenics carrying modified genes and/or transgenes. These mice carry the H-2b haplotype and their immune cells are commonly used in immune-based assays.
Background
CD3 pan T cells from C57BL/6 mice consist of both CD4 and CD8 T cells and make up approximately 33% of cells in the spleen of a typical adult animal. All T cells carry the CD3 protein marker and the T cell receptor (TCR), which recognizes foreign antigens presented by major histocompatibility complex molecules on antigen presenting cells, to activate them.
Species
C57BL/6 Mouse
Cell Type
CD3 Pan T Cells
Features
High quality;
High viability;
Widely applications.
Additional Donor Information
Contact us for donor specifications (Age,Gender,BMI category, smoking status, ethnicity, etc.).
Formulation
Contains Cryopreservation Medium
Cell Purity
>95%
Cell Viability
>90%
Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Sterility Testing
Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Genetic Stability Testing
We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.
Application
Immunotherapy Discovery and Development
Research Use Only
Our immune cell are for research use only, not for diagnostic or therapeutic use.
Quality Control
Cultures are screened for the presence of bacteries, yeast, fungi and mycoplasma (DNA amplification). Growth media are also certified based on U.S. Public Health Service Guidelines.
Storage
Lyophilized cells should be stored in a liquid nitrogen tank (-150°C~-190°C). After thawing, cells should be immediately transferred to the appropriate culture medium and maintained under recommended culture conditions.
Handling Notes
Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.
Warnings
Avoid multiple freeze/thaw cycles
Size
1 x 10e6 cells/vial
Status
Frozen
Note
Certain products are only available in select territories. Please contact us for more information.
For Research Use Only | Not For Clinical Use
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