MetaVital™ Mouse Dimethylaniline monooxygenase [N-oxide-forming] 4 (FMO4) ELISA Kit

CAT#: ITS-0326-WB19
Product Type: ELISA Kit
Species: Mouse
Target: Mouse Dimethylaniline monooxygenase [N-oxide-forming] 4 (FMO4) protein
Brand: MetaVital™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Quantitatively measures FMO4 enzyme levels in mouse tissues, cells, and biological fluids with high sensitivity.
Brand
MetaVital™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
MetaVital™ Mouse FMO4 ELISA Kit enables precise in vitro quantification of Dimethylaniline monooxygenase [N-oxide-forming] 4 in tissue homogenates, cell lysates, and other biological samples. Its colorimetric detection provides reproducible results, supporting research in xenobiotic metabolism and enzymatic regulation studies.
Features
Sensitive colorimetric assay detecting FMO4 concentrations ranging from 0.156-10 ng/mL in diverse mouse samples.
Optimized for tissue homogenates, cell lysates, and biological fluids, ensuring versatile sample compatibility.
Lyophilized standards allow reliable quantification with minimal variability and extended shelf-life stability.
Kit demonstrates <5% activity loss under recommended storage, ensuring consistent, reproducible experimental performance.
Process Relevance
Monitors FMO4 activity to support studies of xenobiotic metabolism and enzyme regulation in preclinical research.
Application Stage
Drug metabolism research, pharmacokinetics, and preclinical enzyme activity studies.
Applications
Applicable for quantitative ELISA measurements in mouse tissue, cell lysates, or biological fluids, supporting metabolic profiling, toxicity studies, ADME profiling studies, and enzyme expression analysis.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
Mouse Dimethylaniline monooxygenase [N-oxide-forming] 4 (FMO4) protein
Detection Method
Colorimetric
Sample Type
Tissue homogenates, cell lysates and other biological fluids.
Assay Measures
Quantitative
Research Areas
Xenobiotic metabolism, enzymology, pharmacokinetics (ADME), toxicology, preclinical drug development, etc.
Size
96 tests
Data Output Type
Quantitative enzyme concentration measurement using colorimetric detection (endpoint assay).
Assay Range
0.156 ng/ml - 10 ng/ml
Species
Mouse
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
FMO4 is a flavin-containing monooxygenase involved in the oxidative metabolism of xenobiotics and endogenous compounds. Its activity influences drug biotransformation, detoxification, and oxidative stress responses, making it a critical target in pharmacological and toxicological research.
Pathways
FMO4 catalyzes N-oxidation reactions of primary and secondary amines, upstream of hepatic clearance and downstream of xenobiotic absorption. It interacts with NADPH-dependent electron transfer and other phase I metabolic enzymes, modulating overall oxidative metabolism.
For Research Use Only | Not For Clinical Use
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