T & B Cell based ELISPOT Assay Service
Creative Biolabs provides the advanced Enzyme-Linked Immunospot (ELISPOT) assay to gain an insight into antigenic epitopes by measuring cytokine secretion activity. Creative Biolabs provides a powerful solution for monitoring cellular immune responses with unmatched sensitivity and precision. We help you quantify antigen-specific cells, assess the functional output of your immune models, and gain critical insights for your research. Our service delivers actionable data that can accelerate your projects from basic research to clinical trials.
Introduction What We Can Offer How We Can Help Why Creative Biolabs Customer Reviews FAQs Related Services Contact Us
Enzyme-linked Immune Absorbent Spot Assay for Cancer Epitope Analysis
Investigation of antigenic peptide-specific humoral and cellular immunity can be monitored by the detection of cell activation response, such as cell expansion, cell-specific killing, and cytokine and other effector molecules production. By measuring cytokine concentration changes in PBMC, we can directly validate T cell stimulation by specific antigen peptides. Due to the accurate, sensitive, reproducible results of ELISPOT, the assay became the gold standard for evaluating antigen-specific immune response since it was developed and is widely used for various studies, including CTL monitoring, viral antigen-related cell response diagnosis in clinical experiments, and vaccine candidates.
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Fig.1 Principle of ELISPOT Assay.
What We Can Offer
Assay Validation and Optimization
We ensure the optimal performance of every trial by meticulously validating the assay parameters. This includes optimizing starting cell numbers, specific antigen concentrations, and antibody concentrations for your specific PBMC or spleen cell samples.
Data Analysis
Our robust, automated image system ensures accurate, objective results by imaging and analyzing color spots for quantitative immune response interpretation. We also offer an optimized two-color ELISPOT assay that uses different colored reactions to discriminate between cell populations producing different cytokines, with an automated report on total and subpopulation spot numbers.
Available ELISPOT Assays
Compared to other methods measuring individual cytokine-secreting cells, the ELISPOT assay always outranks others due to its unique sensitivity to detect low-frequency antigen-specific lymphocytes. ELISPOT can also be used to assess multiple cytokines produced by T-helper (Th) cells, Th2 cells, Th17 cells, and B cells. The popular ELISPOT assay includes, but is not limited to below.
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Popular ELISPOT Assay Targets
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IFN-γ
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IL-5
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Granzyme B
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IL-10
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TNF
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IL-4
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IL-13
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FasL
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IL-1 beta
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IL-17
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IL-17A
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IL-17F
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Perforin
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TNFa
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IL-2
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IL-12p70
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IL-6
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ELISPOT Assay Services at Creative Biolabs
ELISPOT is typically used to evaluate antigen-specific immune responses of T and B cells. This assay is also reliable in studying cancer epitopes that can elicit host immune responses. With the mature and formal platform developed by our in-house talented experts and technicians, Creative Biolabs offers a highly sensitive T and B cell-based ELISPOT assay for cancer epitope analysis for our clients.
Workflow
The ELISPOT assay is ideal for measuring both immunoglobulins secreted by epitope-specific B cells and the cytokine release of specific epitope-stimulated T cells. The procedure contains the following main steps:
Highlights
High Sensitivity
Our method allows for the detection of rare antigen-specific lymphocytes, with the ability to identify a single cytokine-producing cell out of 105 cells in a culture. This unmatched sensitivity is critical for early-stage immune response detection.
High-throughput
The assay is designed for efficiency, enabling the simultaneous analysis of numerous samples in a single trial. This high-throughput capability is ideal for immune kinetic studies and large-scale screening projects.
Quantitative Measurement
Our technique provides a wide quantitative range for measuring cytokine and antibody production, giving you a precise and reliable measure of cellular activity.
Functional Measurement
Our ELISPOT assay uniquely provides a direct measurement of cellular function by quantifying the specific molecules actively secreted by individual, antigen-stimulated cells, a capability that distinguishes it from bulk assays.
Discover how Creative Biolabs' T and B cell-based ELISPOT assay can streamline your research - Get a quote today.
Customer Reviews
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Efficient Lead Screening
Using Creative Biolabs' assay in our research has significantly improved our ability to screen potential drug candidates. The high-throughput capability allowed us to test a large number of compounds quickly. - S** G*******.
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Precise Antibody Characterization
The detailed data on immunoglobulin isotypes provided by the ELISPOT assay were a game-changer for our antibody development work. We were able to confirm isotype switching and fine-tune our immunization strategy. - L** S*******.
FAQs
How is ELISPOT different from a standard ELISA?
While both are immunoassays, a standard ELISA measures the total concentration of a molecule in a sample (e.g., a cell supernatant), while ELISPOT provides single-cell resolution. It quantifies the exact number of active, secreting cells in your population, which is crucial for assessing function.
Can your ELISPOT assay be used for my specific disease model?
Yes, our assay is highly adaptable. We have extensive experience with various applications, including oncology, infectious diseases, and autoimmune disorders. We can use a variety of cell types and antigens to meet the specific needs of your project.
Related Services
Immuno-oncology Flow Cytometry Assay Service
We offer a high-throughput, sensitive, and versatile flow cytometry platform for epitope binning.
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Antibody and Protein Production
We provide comprehensive services for the production of high-quality proteins and antibodies necessary for your ELISPOT and other assays.
Learn More →
How to Contact Us
Creative Biolabs is your trusted partner in immunological research. We offer cutting-edge T and B Cell-based ELISPOT assays designed to accelerate your discovery process and provide you with precise, actionable data. With our expertise and comprehensive services, you can move your research forward with confidence.
To learn more about our T and B cell-based ELISPOT assay and to discuss your specific project needs, please reach out to our team now.
