We provide a highly efficient, DNA-encoded epitope library for rapid T cell-based reporter gene assays. This system significantly reduces research costs while enabling the screening of thousands of samples in a single, streamlined process.
T Cell based Reporter Gene Assay Service
Creative Biolabs provides a powerful and precise solution for evaluating T cell function. Our platform is engineered to deliver specific, actionable data that directly addresses the core challenges in immuno-oncology research, from target identification to therapy validation. Creative Biolabs provides clear, quantitative measurements of T cell activation in response to a wide range of stimuli, including small molecules, biologics, and cellular antigens.
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Applications of Reporter Gene-based T-Cell Assay
Reporter gene-encoded protein is used by researchers to indicate the expression of the gene of interest, expressed naturally or a transgenic gene fusion to the target genome, successfully. The reporter gene is widely employed in various studies on cell-level and animal levels to explain the complex process. IL-2 secretion is a selective marker for activated T cells and is often used as an indicator of antigen-specific T cell activation. Other reporter genes, such as GFP and luciferase, are integrated under the control of human promoters to monitor T cell responses.
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What We Can Offer
High-Throughput Screening
Comprehensive Epitope Analysis
Our platform allows for the simultaneous testing of MHC class I and II with various HLA alleles. This, combined with next-generation sequencing, provides a full, comprehensive analysis of antigenic epitopes.
Unambiguous Readouts
We eliminate the complexity of endogenous T cell receptors by using proprietary TCR-knockout Jurkat cell lines. This results in cleaner, more reliable data with a higher signal-to-noise ratio.
Versatile Applications
Our platform extends beyond drug screening, enabling the selection of immunogenic epitopes from pathogens, the isolation of specific T cells, and the identification of epitope-binding TCR sequences.
Reporter Gene-based Epitope Analysis Services at Creative Biolabs
Creative Biolabs provides various bioassays for cancer epitope analysis and assessment. Here, we present a reporter gene-based T-cell assay to identify class I- and class II-restricted epitopes.
Highlights
Expert Partnership
Partner with our dedicated team of experts to accelerate your immuno-oncology research. We are committed to providing superior specificity, reliability, and high-throughput capabilities to help you achieve your project goals efficiently and confidently.
Advanced Technology
Our service stands out by utilizing proprietary TCR-KO cell lines and advanced dual-reporter systems. These technologies are engineered to deliver data that is far more accurate and meaningful than what is possible with conventional assay methods.
Clean Data Signals
We have specifically designed our platform to eliminate common challenges like false positives and background noise. This ensures that every readout is a cleaner and more precise signal, giving you a higher degree of confidence in your results.
Unrivaled Reliability
Our robust systems offer superior specificity and reliability. This means you can trust our data to be both reproducible and actionable, empowering you to make critical decisions with confidence throughout your drug discovery and development pipeline.
Discover how a partnership with us can streamline your research - Get a quote today.
Customer Reviews
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Reliable and Actionable Data
Using Creative Biolabs' assay in our research has significantly improved our ability to screen for low-avidity TCR-antigen interactions. The dual-reporter system provides an essential internal control, giving us confidence in our results. - Dr. T***a R. -
High-Throughput Screening
This service has completely changed our high-throughput screening workflow. The platform's sensitivity and the speed of the "add-mix-read" protocol have facilitated our lead compound identification process. - A***n M.
FAQs
How do T Cell-based reporter gene assays compare to traditional methods like ELISA or ELISPOT?
Our reporter gene assays offer superior sensitivity and are more amenable to high-throughput screening. The assays provide a real-time, quantitative readout of an early T cell signaling event, allowing for more precise data on compound potency and kinetics.
What if my compound is cytotoxic? Will it interfere with the assay results?
Our advanced dual-reporter system is specifically designed to address this concern. The second, constitutively expressed reporter provides an internal control for cell viability, allowing us to normalize the primary signal and distinguish between true inhibition and compound cytotoxicity.
Related Services
T Cell Engineering Services
We provide comprehensive services for the design, generation, and functional characterization of engineered T cells, including CAR-T and TCR-T cell therapies.
Learn More →Immune Checkpoint Assays
We provide a comprehensive service for assessing the functional activity of immune checkpoint inhibitors, including PD-1, PD-L1, and CTLA-4. Our assays are ideal for preclinical studies and for guiding the development of novel antibodies.
Learn More →How to Contact Us
Creative Biolabs is an oncology research consultancy and experimental service provider. With many years of experience in cancer study, Creative Biolabs offers clients the most suitable solutions with premium quality and efficiency. Please contact us for more information.
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