Vesitra™ Efflux Transporter Cell Panel

A specialized suite of stable MDCK-II cell lines expressing critical human ABC transporters, optimized for bidirectional transport assays and efflux ratio determination.

Vesitra™

ADME

Metabolism; Absorption; Excretion

The Vesitra™ Efflux Transporter Cell Panel is specifically engineered for assessing transcellular transport and identifying efflux liability. Featuring critical ABC transporters like P-gp (MDR1) and BCRP, along with a matched Mock control, this panel utilizes the well-characterized MDCK-II host cell line. MDCK-II cells are recognized as the industry standard for these assays because they rapidly form polarized monolayers with tight junctions while maintaining an optimally low baseline transepithelial electrical resistance (TEER). This makes the panel an ideal in vitro model for mimicking the human intestinal barrier and the blood-brain barrier (BBB) to accurately evaluate drug efflux.

1. Optimized Host Cell: Utilizes the MDCK-II strain to ensure optimal baseline TEER values and low background passive permeability.
2. High Efflux Ratios: Consistently delivers robust and reproducible Efflux Ratios for standard reference substrates.
3. Excellent Monolayer Integrity: Readily forms tight, fully polarized monolayers on standard transwell inserts within a short culture period.
4. Mycoplasma-Free Guarantee: Rigorously tested to ensure high viability, sterility, and absence of microbial contamination.

1. Bidirectional transcellular transport assays.
2. Determination of apparent permeability and functional Efflux Ratios.
3. Evaluation of intestinal absorption potential and Blood-Brain Barrier penetration.
4. Identification of efflux transporter substrates and characterization of potent inhibitors to assess DDI risks.

Madin-Darby Canine Kidney

Stable Transfection

Canine

MDCK

Kidney

This specialized bundle utilizes polarized MDCK-II cells to create a robust in vitro barrier model. Featuring the critical ABC transporters P-gp and BCRP, it is the industry gold standard for bidirectional transcellular transport assays. It empowers researchers to definitively identify efflux liabilities, assess intestinal absorption potential, and evaluate Blood-Brain Barrier (BBB) penetration.

2×10⁶ / vial lyophilized cells

>95%

>90%

Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.

A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus saftey assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.

We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Dry Ice

Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C). Once reconstituted, the cells may be used for up to five days if properly stored at 2°C - 8°C in the buffer provided.

Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.

Avoid multiple freeze/thaw cycles

For research use only, not for diagnostic or therapeutic use.

All of our stable cell lines meet the highest quality standards and have been confirmed to be pathogen-free.