Vesitra™ Human OATP1A2 Stable HEK293 Cell Line

CAT#: ITS-0326-HM4
Product Type: Transporter Cell Line
Organism: Human
Brand: Vesitra™
Development Stage: ADME
ADME Research Domain: Metabolism; Absorption; Excretion
Short Description
The ideal in vitro OATP1A2 model for penetrating the blood-brain barrier (BBB) and multi-tissue distribution research.
Brand
Vesitra™
Development Stage
ADME
ADME Research Domain
Metabolism; Absorption; Excretion
Description
Our Vesitra™ Human OATP1A2 expression line, constructed via high-fidelity amplification, stably presents the transporter's folded conformation on the membrane surface. Designed for CNS-targeted drugs or compounds with broad tissue distribution, it provides clear non-hepatic OATP uptake kinetics.
Features
1. Efficiently simulates OATP-mediated uptake across multiple sites (BBB, kidneys, intestines). 2. Serum-free tolerance allows for>4 hours of viability in assay buffers, eliminating protein-binding concentration errors.
Applications
Permeability evaluation of Central Nervous System (CNS) drugs; Tissue distribution and toxicity prediction for antihistamines.
Parental Cell Line
Human Embryonic Kidney 293 (HEK293)
Transfection
Stable Transfection
Transporter
OATP1A2
Gene
SLCO1A2
Typical Probe Substrate
Estrone-3-sulfate (E3S) / Fexofenadine
Reference Inhibitor
Rifampin (Rifampicin) / Naringin
Organism
Human
Strain Name
HEK293
Tissue
Embryonic Kidney
Background
OATP1A2 (SLCO1A2) is widely distributed across the liver, kidney, intestine, and the blood-brain barrier (BBB). It plays a significant role in the systemic distribution and tissue-specific penetration of various xenobiotics, including antihistamines (e.g., fexofenadine) and antivirals, serving as a key model for evaluating drug distribution pharmacokinetics.
Morphology
Epithelial
Unit Size
2×10⁶ / vial lyophilized cells
Culture Properties
Adherent
Cell Purity
>95%
Cell Viability
>90%
Sterility Testing
Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Identity Testing
Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.
Virological Safety Testing
A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus saftey assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.
Genetic Stability Testing
We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.
Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Shipping
Dry Ice
Storage
Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C). Once reconstituted, the cells may be used for up to five days if properly stored at 2°C - 8°C in the buffer provided.
Handling Notes
Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.
Warnings
Avoid multiple freeze/thaw cycles
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Quality Control
All of our stable cell lines meet the highest quality standards and have been confirmed to be pathogen-free.
For Research Use Only | Not For Clinical Use
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