Vesitra™ Mouse MDR1 Stable MDCK Cell Line

CAT#: ITS-0326-HM30
Product Type: Transporter Cell Line
Organism: Canine
Brand: Vesitra™
Development Stage: ADME
ADME Research Domain: Metabolism; Absorption; Excretion
Short Description
A polarized MDCK cell model stably expressing murine MDR1, essential for preclinical mouse pharmacokinetic and BBB permeability evaluations.
Brand
Vesitra™
Development Stage
ADME
ADME Research Domain
Metabolism; Absorption; Excretion
Description
Designed to bridge the translational gap between in vitro assays and murine in vivo models. The Vesitra™ Mouse MDR1 cell line forms intact epithelial monolayers, allowing for accurate bidirectional transport measurements to predict intestinal absorption and brain penetration specifically in mouse models.
Features
1. Expresses the predominant murine P-gp ortholog to ensure species-specific substrate recognition. 2. Generates robust polarized monolayers suitable for Transwell permeability setups. 3. Exhibits excellent correlation with in vivo murine brain-to-plasma ratios.
Applications
Predicting murine blood-brain barrier (BBB) penetration; Assessing species differences in P-gp-mediated efflux before initiating mouse efficacy and toxicity models.
Parental Cell Line
Madin-Darby Canine Kidney
Transfection
Stable Transfection
Transporter
Mdr1a/1b
Gene
Abcb1a/1b
Typical Probe Substrate
Digoxin / Rhodamine 123
Reference Inhibitor
Verapamil / Valspodar
Organism
Canine
Strain Name
MDCK
Tissue
Kidney
Background
Mouse Mdr1 (Mdr1a/1b) expresses the murine ortholog of human P-gp. While human clinical data is paramount, early-stage in vivo pharmacokinetic and toxicology studies heavily rely on murine models. This cell line provides the essential in vitro functional data required to understand species-specific efflux kinetics and accurately bridge mouse preclinical data to human outcomes.
Morphology
Epithelial
Unit Size
2×10⁶ / vial lyophilized cells
Culture Properties
Adherent
Cell Purity
>95%
Cell Viability
>90%
Sterility Testing
Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Identity Testing
Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.
Virological Safety Testing
A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus saftey assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.
Genetic Stability Testing
We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.
Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Shipping
Dry Ice
Storage
Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C). Once reconstituted, the cells may be used for up to five days if properly stored at 2°C - 8°C in the buffer provided.
Handling Notes
Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.
Warnings
Avoid multiple freeze/thaw cycles
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Quality Control
All of our stable cell lines meet the highest quality standards and have been confirmed to be pathogen-free.
For Research Use Only | Not For Clinical Use
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