Vesitra™ Uptake Transporter Cell Panel

CAT#: ITS-0326-HM43
Product Type: Cell Panel
Organism: Human
Brand: Vesitra™
Development Stage: ADME
ADME Research Domain: Metabolism; Absorption; Excretion
Short Description
A ready-to-use, comprehensive bundle of stable HEK293 cell lines expressing key human SLC transporters, designed for high-throughput uptake assays and regulatory DDI screening.
Brand
Vesitra™
Development Stage
ADME
ADME Research Domain
Metabolism; Absorption; Excretion
Description
The Vesitra™ Uptake Transporter Cell Panel provides a complete, standardized toolkit for evaluating the active cellular uptake of drug candidates. This panel includes major hepatic and renal transporters-such as OATPs, OATs, OCTs, and MATEs-alongside a matched Mock HEK293 control cell line. Engineered for robust and highly stable expression, these cell lines allow researchers to reliably identify substrate specificities and inhibitor potencies. By utilizing a uniform HEK293 background, this panel minimizes assay variability, facilitating accurate predictions of in vivo tissue clearance and potential Drug-Drug Interactions (DDIs).
Features
1. Robust Signal Window: Delivers excellent signal-to-noise ratios (typically ≥ 5-fold uptake compared to Mock cells) for confident data interpretation.
2. Genetic Stability: Functional expression and robust activity are strictly validated to remain stable for up to 15-20 passages post-thaw.
3. Stringent Quality Control: Each lot is pre-validated with typical probe substrates and reference inhibitors, ensuring consistent lot-to-lot performance.
4. Isogenic Background: All transporters are expressed in the same HEK293 host lineage, allowing for direct and reliable cross-target comparisons.
Applications
1. ADME and pharmacokinetic (PK) profiling of new chemical entities (NCEs).
2. Intracellular uptake assays and determination of enzyme kinetic parameters.
3. Screening for potential drug-drug interactions.
Parental Cell Line
Human Embryonic Kidney 293 (HEK293)
Transfection
Stable Transfection
Organism
Human
Strain Name
HEK293
Tissue
Embryonic Kidney
Background
Assessing potential Drug-Drug Interactions (DDIs) requires investigating multiple cellular pathways. This bundled panel provides a ready-to-use, standardized platform encompassing key hepatic and renal SLC transporters. It allows for rapid, parallel, high-throughput screening to accurately predict in vivo clearance and metabolic liabilities of candidate drugs.
Unit Size
2×10⁶ / vial lyophilized cells
Cell Purity
>95%
Cell Viability
>90%
Sterility Testing
Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Identity Testing
Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.
Virological Safety Testing
A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus saftey assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.
Genetic Stability Testing
We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.
Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Shipping
Dry Ice
Storage
Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C). Once reconstituted, the cells may be used for up to five days if properly stored at 2°C - 8°C in the buffer provided.
Handling Notes
Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.
Warnings
Avoid multiple freeze/thaw cycles
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Quality Control
All of our stable cell lines meet the highest quality standards and have been confirmed to be pathogen-free.
For Research Use Only | Not For Clinical Use
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