Vesitra™ Human OATP1B3 Stable HEK293 Cell Line

CAT#: ITS-0326-HM2
Product Type: Transporter Cell Line
Organism: Human
Brand: Vesitra™
Development Stage: ADME
ADME Research Domain: Metabolism; Absorption; Excretion
Short Description
A stable OATP1B3 expression model for accurately simulating the uptake of macromolecular and peptide drugs at the hepatocyte basolateral membrane.
Brand
Vesitra™
Development Stage
ADME
ADME Research Domain
Metabolism; Absorption; Excretion
Description
Vesitra™ Human OATP1B3 utilizes proprietary vector technology to achieve dense expression of this macromolecular transporter on the HEK293 cell membrane. Complementary to OATP1B1, it is an essential in vitro tool for assessing the hepatic first-pass effect, with a substrate preference for larger hydrophobic anions.
Features
1. Precise substrate recognition validated by specific substrates/inhibitors (e.g., CCK-8, Rifampicin) with zero off-target background. 2. Rapid and uniform monolayer formation, perfectly suited for automated 96/384-well high-throughput washing.
Applications
Hepatic uptake assessment for targeted macromolecule/peptide drugs; Deciphering complex DDI mechanisms co-mediated by OATP1B1 and 1B3.
Parental Cell Line
Human Embryonic Kidney 293 (HEK293)
Transfection
Stable Transfection
Transporter
OATP1B3
Gene
SLCO1B3
Typical Probe Substrate
CCK-8 / Estradiol-17β-D-glucuronide
Reference Inhibitor
Rifampin (Rifampicin) / Cyclosporin A
Organism
Human
Strain Name
HEK293
Tissue
Embryonic Kidney
Background
OATP1B3 (SLCO1B3) is a liver-specific uptake transporter expressed on the basolateral membrane of hepatocytes. Working in tandem with OATP1B1, it mediates the hepatic influx of endogenous compounds (like bile acids) and various therapeutic drugs. Assessment of OATP1B3 inhibition or substrate potential is an essential component of comprehensive pharmacokinetic evaluations.
Morphology
Epithelial
Unit Size
2×10⁶ / vial lyophilized cells
Culture Properties
Adherent
Cell Purity
>95%
Cell Viability
>90%
Sterility Testing
Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Identity Testing
Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.
Virological Safety Testing
A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus saftey assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.
Genetic Stability Testing
We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.
Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Shipping
Dry Ice
Storage
Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C). Once reconstituted, the cells may be used for up to five days if properly stored at 2°C - 8°C in the buffer provided.
Handling Notes
Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.
Warnings
Avoid multiple freeze/thaw cycles
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Quality Control
All of our stable cell lines meet the highest quality standards and have been confirmed to be pathogen-free.
For Research Use Only | Not For Clinical Use
Online Inquiry