Vesitra™ Human PEPT2 Stable HEK293 Cell Line

CAT#: ITS-0326-HM14
Product Type: Transporter Cell Line
Organism: Human
Brand: Vesitra™
Development Stage: ADME
ADME Research Domain: Metabolism; Absorption; Excretion
Short Description
A high-sensitivity PEPT2 model focused on peptide reabsorption studies in the kidneys and central nervous system.
Brand
Vesitra™
Development Stage
ADME
ADME Research Domain
Metabolism; Absorption; Excretion
Description
Complementary to the intestinal PEPT1, this product focuses on high-affinity peptide transport in the renal proximal tubules and choroid plexus. This cell line demonstrates a lower Km value, making it ideal for studying the long-term circulation mechanisms of trace peptide drugs in vivo.
Features
1. Extremely high substrate affinity suitable for low-concentration drug transport assays. 2. Robust cell adhesion supporting extended incubation experiments. 3. Strict functional consistency with batch-to-batch CV values < 15%.
Applications
Predicting renal reabsorption capacity for peptide drugs; Investigating peptide pathways across the blood-brain or blood-cerebrospinal fluid barriers.
Parental Cell Line
Human Embryonic Kidney 293 (HEK293)
Transfection
Stable Transfection
Transporter
PEPT2
Gene
SLC15A2
Typical Probe Substrate
Glycylsarcosine (Gly-Sar)
Reference Inhibitor
Cefadroxil
Organism
Human
Strain Name
HEK293
Tissue
Embryonic Kidney
Background
PEPT2 (SLC15A2) is a high-affinity, low-capacity peptide transporter predominantly expressed on the apical membrane of the renal proximal tubule. It is responsible for the efficient reabsorption of small peptides and peptide-like drugs from the urinary filtrate back into systemic circulation, thereby significantly influencing their biological half-life.
Morphology
Epithelial
Unit Size
2×10⁶ / vial lyophilized cells
Culture Properties
Adherent
Cell Purity
>95%
Cell Viability
>90%
Sterility Testing
Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Identity Testing
Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.
Virological Safety Testing
A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus saftey assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.
Genetic Stability Testing
We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.
Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Shipping
Dry Ice
Storage
Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C). Once reconstituted, the cells may be used for up to five days if properly stored at 2°C - 8°C in the buffer provided.
Handling Notes
Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.
Warnings
Avoid multiple freeze/thaw cycles
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Quality Control
All of our stable cell lines meet the highest quality standards and have been confirmed to be pathogen-free.
For Research Use Only | Not For Clinical Use
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