BiliTrack™ Cow Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) ELISA Kit

CAT#: ITS-0326-WB149
Product Type: ELISA Kit
Species: Cow
Target: Bovine tissue-nonspecific alkaline phosphatase (ALPL) enzyme
Brand: BiliTrack™
Development Stage: ADME
ADME Research Domain: Excretion
Short Description
Quantifies bovine ALPL levels in biological samples using a sensitive sandwich ELISA method.
Brand
BiliTrack™
Development Stage
ADME
ADME Research Domain
Excretion
Description
BiliTrack™ Cow Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) ELISA Kit is a robust immunoassay designed for accurate quantification of ALPL in bovine samples. Based on a highly specific sandwich ELISA format, this kit enables reproducible detection in serum, plasma, and tissue lysates, supporting studies in bone biology, liver physiology, and metabolic regulation.
Features
Highly specific antibody pairing enables precise detection of bovine ALPL with minimal cross-reactivity in complex biological sample matrices.
Sensitive colorimetric assay design supports reliable quantification across a broad dynamic concentration range for diverse experimental needs.
Complete kit components including pre-coated plates and standards ensure streamlined workflows and reproducible inter-assay performance.
Compatible with multiple bovine sample types, providing flexibility for varied research applications in physiology and disease modeling.
Process Relevance
Enables accurate monitoring of ALPL levels to assess metabolic and tissue-specific enzymatic activity changes.
Application Stage
Veterinary research, biomarker validation, and preclinical physiological studies.
Applications
Quantification of ALPL in bovine serum, plasma, and tissues; evaluation of bone turnover; liver function studies; ADME profiling studies; enzymatic activity assays; biomarker analysis in disease models.
Target
Bovine tissue-nonspecific alkaline phosphatase (ALPL) enzyme
Detection Method
Colorimetric
Sample Type
Tissue homogenates, cell lysates and other biological fluids.
Assay Measures
Quantitative
Research Areas
Veterinary science, bone metabolism, hepatology, enzymology, pharmacokinetics (ADME), metabolic disease research, etc.
Size
96 tests
Data Output Type
Quantitative measurement of ALPL protein concentration via colorimetric ELISA detection.
Assay Range
0.156 ng/ml - 10 ng/ml
Species
Cow
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Alkaline phosphatase (ALPL) is a key hydrolase enzyme involved in dephosphorylation and mineralization processes. It is abundantly expressed in liver and bone tissues, where it regulates phosphate availability. Dysregulation of ALPL is associated with skeletal abnormalities and hepatobiliary dysfunction.
Pathways
ALPL functions within phosphate metabolism and biomineralization pathways, contributing to hydroxyapatite formation during bone development. It is regulated by osteoblast differentiation signals and reflects liver metabolic activity. Its enzymatic activity also influences extracellular matrix remodeling and systemic phosphate balance.
For Research Use Only | Not For Clinical Use
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