CytoFinal™ Low Sample Volume Mouse Aspartate Aminotransferase, Cytoplasmic / AST (GOT1) ELISA Kit

CAT#: ITS-0326-WB94
Product Type: ELISA Kit
Species: Mouse
Target: Cytoplasmic Aspartate Aminotransferase (AST/GOT1)
Brand: CytoFinal™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Measures cytoplasmic AST (GOT1) in mouse samples using minimal volumes for high-precision metabolic and hepatocellular studies.
Brand
CytoFinal™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
CytoFinal™ Low Sample Volume Mouse AST (GOT1) ELISA Kit enables accurate detection of cytoplasmic AST from small-volume mouse serum, plasma, tissue, or cell lysates. Designed for low sample requirements, it supports preclinical research and hepatocellular integrity evaluation with high sensitivity and reproducibility.
Features
Minimal sample volume requirement allows precise cytoplasmic AST measurement in limited mouse serum or tissue samples.
High specificity ensures selective detection of cytoplasmic AST without interference from mitochondrial isoforms.
Standardized reagents provide reproducible results across various sample types and experimental conditions.
Rapid colorimetric assay enables efficient processing of multiple samples with reliable low-level AST quantification.
Process Relevance
Facilitates sensitive cytoplasmic AST assessment in small-volume mouse samples, improving hepatocellular monitoring and metabolic research.
Application Stage
Applicable in preclinical research, pharmacology studies, toxicology testing, and low-volume laboratory experiments.
Applications
Sensitive quantification of cytoplasmic AST in mouse serum, plasma, tissues, or cell lysates for hepatotoxicity evaluation and ADME profiling studies.
Target
Cytoplasmic Aspartate Aminotransferase (AST/GOT1)
Detection Method
Colorimetric
Sample Type
Serum, plasma, tissue homogenates and other biological fluids.
Assay Measures
Quantitative
Research Areas
Hepatology, preclinical pharmacology, metabolism, pharmacokinetics (ADME), enzymology, biomarker discovery, toxicology, translational research, etc.
Size
96 tests
Data Output Type
Quantitative colorimetric measurement of cytoplasmic AST levels in small-volume mouse samples.
Assay Range
31.2 pg/ml - 2000 pg/ml
LOD
< 13.9 pg/ml
Species
Mouse
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
AST/GOT1 catalyzes reversible transamination between aspartate and α-ketoglutarate, linking amino acid metabolism to energy production. Cytoplasmic AST levels provide insight into hepatocellular integrity, metabolic flux, and preclinical safety assessments.
Pathways
AST participates in the malate-aspartate shuttle, regulating cytoplasmic-mitochondrial redox balance. It contributes to TCA cycle activity, amino acid homeostasis, and energy metabolism in metabolically active cells.
For Research Use Only | Not For Clinical Use
Online Inquiry