CytoFinal™ Mouse Lactate Dehydrogenase A (LDHA) ELISA Kit

CAT#: ITS-0326-WB111
Product Type: ELISA Kit
Species: Mouse
Target: Mouse Lactate Dehydrogenase A (LDHA)
Brand: CytoFinal™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Quantitative ELISA kit for detecting mouse Lactate Dehydrogenase A (LDHA) in biological samples.
Brand
CytoFinal™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
CytoFinal™ Mouse Lactate Dehydrogenase A (LDHA) ELISA Kit enables precise quantification of LDHA protein levels in mouse-derived biological samples. Utilizing a sensitive sandwich ELISA format with HRP-based colorimetric detection, this assay supports reliable measurement of LDHA in serum, plasma, tissue lysates, and cell culture extracts for metabolic and disease research applications.
Features
Highly specific sandwich ELISA format employing validated antibody pairs enables accurate detection of mouse LDHA with minimal background interference.
Optimized assay sensitivity and broad linear detection range support reliable quantification across diverse biological sample matrices.
HRP-TMB colorimetric detection system generates stable signals compatible with standard microplate readers for efficient laboratory workflows.
Complete kit configuration including precoated plates, calibrated standards, and optimized reagents ensures reproducible results and streamlined experimental procedures.
Process Relevance
Measures LDHA protein levels to investigate metabolic reprogramming and glycolytic activity in experimental models.
Application Stage
Metabolic research, oncology studies, biomarker discovery, and preclinical therapeutic evaluation.
Applications
Quantification of mouse LDHA in serum, plasma, tissue lysates, and cultured cells for metabolic pathway analysis, tumor metabolism studies, hypoxia research, ADME profiling studies, and therapeutic response assessment.
Target
Mouse Lactate Dehydrogenase A (LDHA)
Detection Method
Colorimetric
Sample Type
Tissue homogenates, cell lysates and other biological fluids.
Assay Measures
Quantitative
Research Areas
Cancer metabolism, glycolytic pathway research, hypoxia biology, metabolic disease studies, pharmacokinetics (ADME), immunometabolism, toxicology, etc.
Size
96 tests
Data Output Type
Quantitative colorimetric measurement of LDHA protein concentration determined by ELISA absorbance.
Assay Range
0.78 ng/ml - 50 ng/ml
LOD
< 0.39 ng/ml
Species
Mouse
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Lactate dehydrogenase A (LDHA) is a major isoform of lactate dehydrogenase that catalyzes the conversion of pyruvate to lactate during glycolysis. It plays a crucial role in maintaining cellular energy production under hypoxic conditions. Increased LDHA expression is commonly associated with metabolic reprogramming in rapidly proliferating cells and various pathological conditions.
Pathways
LDHA functions in the glycolytic pathway by converting pyruvate into lactate while regenerating NAD+ required for continuous glycolysis. This reaction is particularly important during hypoxia or high metabolic demand. LDHA activity is closely linked with hypoxia-inducible factor (HIF-1α) signaling and metabolic reprogramming pathways that support tumor growth and cellular adaptation to low oxygen environments.
For Research Use Only | Not For Clinical Use
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