In vitro ADME screening assays for therapeutic antibodies and proteins are critical for evaluating the pharmacokinetic properties of these biologics. These assays help predict how the antibodies or proteins will behave in the body, aiding in drug development and optimization. Our team of highly skilled scientists is dedicated to providing the most accurate and reliable results possible. We understand that each therapeutic antibody or protein is unique, and therefore, we tailor our assays to meet the specific requirements of your project. Whether you need to assess absorption, distribution, metabolism, or excretion (ADME) properties, we have the expertise and technology to deliver the answers you need.

Fig.1 Schematic of ADME.¹

Our In Vitro ADME Assays

Our common in vitro ADME screening assays include:

• Physicochemical Property
  Solubility assessment is crucial in drug development as the solubility can greatly impact its bioavailability and therapeutic efficacy. We provide solubility studies to assess the drug solubility in different solvents, pH conditions, temperature ranges, and other parameters to optimize its formulation and bioavailability.
  
  • Kinetic solubility testing
  • Thermodynamic solubility testing
• Permeability
  Permeability assay is a type of drug absorption analysis that measure the ability of a drug candidate to cross biological barriers, such as the intestinal epithelium or blood-brain barrier, using cell-based models or artificial membranes. We provide Caco-2 permeability, MDCK-MDR1 permeability, and parallel artificial membrane permeability assay to evaluate the permeability of drugs.
• Metabolic Stability
  Metabolic stability assays assess the drug candidate's metabolic stability and its potential for drug-drug interactions by measuring its metabolism by hepatocyte, liver microsomes, or recombinant enzymes.
• Protein Binding
  These assays evaluate the extent to which a drug candidate binds to plasma proteins, which can impact its bioavailability and distribution in the body.
• Enzyme Inhibition & Enzyme Induction
  Our enzyme inhibition assays evaluate a drug's ability to inhibit enzyme activity, such as cytochrome P450 (CYP) enzymes. We also provide enzyme induction assays to assess a drug's potential to induce enzyme expression. These assays play a critical role in the evaluation of the drug-drug interactions.
• Reaction phenotyping
  Our reaction phenotyping assays are used to identify the metabolic enzymes of a particular drug. Our services help researchers determine which enzymes are involved in the drug metabolism, thereby predicting potential drug-drug interactions.
• Transporter
  We provide comprehensive transporter interaction assessment including a variety of essential ABC transporters and SLC transporters.

Contact Us

At Creative Biolabs, we are committed to providing state-of-the-art and effective ADME solutions. Our high-throughput ADME screening platforms are equipped with advanced instrumentation and automated sample-handling systems. Contact us today to learn more about our in vitro ADME screening assays and how we can help you take your therapeutic antibodies or proteins to the next level.

Reference

1. Hasan, Md Rifat, et al. "Application of mathematical modeling and computational tools in the modern drug design and development process." Molecules 27.13 (2022): 4169. Distributed under Open Access license CC BY 4.0, without modification.

For Research Use Only | Not For Clinical Use