Phage Display based Cancer Epitope Analysis Service
Creative Biolabs provides a systematic and streamlined solution for clients aiming to identify and characterize high-affinity binders for challenging targets. Our deliverables include the identification of unique antibody sequences, detailed characterization data, and the delivery of promising lead candidates. Clients can expect a high-quality, efficient service that minimizes the need for extensive in vitro optimization. Our platform is grounded in real-world applications and designed to provide a direct and effective path to lead candidate identification.
Introduction What We Can Offer Workflow Why Creative Biolabs Customer Reviews FAQs Related Services Contact Us
B Cell-based Phage Display for Cancer Epitope Analysis
Phage display is a high-throughput technique that can display billions of foreign peptides on bacteriophage through the fusion of target peptide genes into coat genes. This approach allows the expression of proteins on the surface of phage in a multivalent type of short peptides or limited copies of long peptides. An epitope is the immunogenic determinant of an antigen that is recognized by T cells, B cells, and antibodies. In previous studies, a random peptide library displayed on phage was adopted to screen B-cell epitopes using monoclonal antibodies and polyclonal antibodies immobilized on a support plate or bound on magnetic beads.
For a comprehensive discussion on how our capabilities can assist your project, request a consultation.
Fig.1 Using phage-displayed peptide libraries and biopanning selection to identify antigen-specific epitopes.1
What We Can Offer
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Phage Displayed Peptide Library Construction
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Potential Antigen Epitope Biopanning
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Full-Length Antigen Proteins Acquisition
We use the DNA sequences of the full-length antigen proteins to identify specific immunogenic epitopes. The DNA sequence information can be derived from whole-genome sequencing and RNA-seq of patient biopsies and tumor tissue.
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Peptides Library Synthesis
A large-scale, random, 12-mer peptide library is generated using trinucleotide oligos to avoid stop codons and use amino acids native-like.
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Phage Display
The peptide library is inserted into E. coli via the engineered protein scaffold.
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Antibody Preparation
Dilute serum samples obtained from patients or immunized mice.
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Antibody Incubation
Antibodies are added to plates containing combinational library cells and incubated for hours.Reactive Epitope Separation
Using magnetic beads bound with protein A/G to capture human/mouse serum antibodies that interact with phage-displayed epitopes.
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Cancer-Specific Epitope Identification
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Magnetic separation system and FACs analysis
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Next-generation sequencing
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Several rounds of magnetic affinity separation
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Phage Display-based Cancer Epitope Analysis at Creative Biolabs
Creative Biolabs applies phage display technology to select high-affinity antigenic peptides of an antigen protein from billions of candidates displayed on phage in a recombinant expression library. Subsequently, the candidate epitopes can be further screened and enriched with a highly selective biopanning approach.
Highlights
High-Throughput Assay
Our platform leverages automated, high-throughput biopanning and screening to quickly process vast libraries. This accelerated approach significantly reduces the time required to isolate high-affinity binders, leading to a faster path to your lead candidates.
Genome-Scale Screening
By utilizing naturally matured B-cell libraries, we capture the full diversity of the native immune repertoire. This enables comprehensive, genome-scale screening to discover a wide range of unique antibody clones and identify the optimal lead candidates.
High-Efficiency and Reliability
Our pre-optimized libraries and robust protocols ensure an exceptionally efficient and reliable approach. This reduces the need for extensive in vitro maturation and provides consistently reproducible results, giving you confidence in the quality of the data.
Experience with Challenging Targets
Our team has extensive experience in working with a variety of challenging antigens, including complex membrane proteins. This expertise allows us to customize screening strategies to overcome common obstacles, ensuring a high success rate for even the most difficult targets.
Discover how a partnership with us can streamline your research - Get a quote today.
Customer Reviews
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Tangible Results
Using Creative Biolabs' B cell-based phage display assay in our research has significantly improved the efficiency of our antibody discovery workflow. We were able to identify high-affinity binders for a challenging therapeutic target in a fraction of the time compared to our previous methods. - Dr. Ma Rs.
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Streamlined Process
We appreciated the clear communication and professional guidance provided by the Creative Biolabs team. Their platform streamlined our entire process, from sample submission to final data analysis, which was crucial for meeting our project deadlines. - Ax Hy.
FAQs
How is your B cell-based phage display assay different from traditional phage display?
Our assay is distinct because it utilizes B cells that have already undergone affinity maturation in vivo. This means the antibodies in our libraries are already optimized for high affinity and specificity, unlike traditional methods that rely on naive libraries and require extensive in vitro maturation.
What types of antigens are suitable for this assay?
The platform is highly versatile and can be used with a wide range of antigens, including proteins, peptides, and even whole cells. Our technology is particularly effective for difficult targets like membrane proteins, which are often challenging to work with using other methods.
Related Services
Phage Display Library Screening
We provide comprehensive screening services for a variety of phage display libraries, including immune, naive, and synthetic libraries. This service allows for the identification of novel peptides, antibodies, and other binders for a wide range of targets beyond our B cell-based platform.
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Antibody Humanization Service
We use advanced in silico and in vitro methods to analyze your antibody candidates for potential immunogenicity. This helps predict and mitigate the risk of adverse immune responses in patients, which is a critical step for therapeutic development.
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How to Contact Us
Creative Biolabs is devoted to developing comprehensive, integrated services covering the broad application of phage display technology. We are devoted to providing high-quality antibody and antigen phage display and customized screening services to meet our partners' particular needs and objectives. Please contact us for further information.
Reference
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Wu, Chien-Hsun et al. "Advancement and applications of peptide phage display technology in biomedical science." Journal of biomedical science vol. 23 8. 19 Jan. 2016. Distributed under an Open Access license CC BY 4.0, without modification. https://doi.org/10.1186/s12929-016-0223-x
For Research Use Only | Not For Clinical Use
