The unspecific binding of CD8+ T cells to MHC multimer/tetramer has been diminished through sequence modification of HLA domains, which dramatically elevated the detection specificity of the T cells.
T Cell based Multimer/Tetramer Assay Service
Creative Biolabs' T Cell-based multimer/tetramer assay is a powerful tool designed to provide you with precise, actionable data on antigen-specific T-cell populations. Inspired by the unique binding interaction between TCR and peptide-MHC, Creative Biolabs offers synthesized peptide-MHC multimer/tetramer and its application in cancer epitope analysis. Creative Biolabs provides specific deliverables, solutions, and key problem-solving capabilities to ensure you achieve your research goals.
Introduction What We Can Offer Workflow Why Creative Biolabs Customer Reviews FAQs Related Services Contact Us
Peptide-MHC Tetramer/Multimer
The exquisite specificity of T cell response to peptide/MHC is useful in tracing antigen-specific T cells and analyzing antigenic peptides. But the binding affinity of a single peptide/MHC to its cognate TCR is low and dissociates quickly. Tetramerized MHC molecules loaded with candidate peptides promoted the association of TCR with the complex. When labeling with fluorescent dyes, MHC tetramer/multimer could act as a staining reagent for epitope-specific T cells, enabling the convenient detection by a flow cytometer. Class I and class II tetramer reagents are produced differently and have been used in identifying CD8+ and CD4+ T cells, respectively.
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Fig.1 Self-reactive T-cells have low-affinity T-cell receptors (TCRs).1
What We Can Offer
High Specificity and Reactivity
High Quality and Efficiency
We adopt superior material for tetramer/multimer production, ensuring the high quality and efficiency of the synthesis. With the assurance of strict quality control throughout the whole production process, our MHC multimer/tetramer demonstrates a high specificity and clear discrimination of antigenic epitopes.
A Large Bank of MHC Alleles
With a bank of over 60 types of HLA alleles, we can produce custom tetramers tailored to your specific research objectives. This extensive library ensures a perfect match for your project.
Workflow of Tetramer-Guided Epitope Mapping (TGEM)
Highlights
Unmatched Experience
Our team's two decades of expertise in immunology and assay development ensure every project is handled with precision and scientific integrity. We are your reliable partner for achieving breakthroughs.
Optimized Protocols
We go beyond standard methods by leveraging advanced staining protocols and higher-order dextramers. This enables us to reliably detect even low-affinity T-cell populations, crucial for cancer and autoimmune research.
Streamlined Process
Our streamlined project workflow, from consultation to final report, is designed for maximum efficiency. We provide timely communication and support to ensure your project progresses smoothly and on schedule.
Cutting-Edge Solutions
We continuously innovate to stay ahead of scientific advancements. By incorporating emerging technologies like advanced enrichment procedures and high-multiplexing capabilities, we deliver data with unparalleled depth.
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Customer Reviews
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Exceptional Customization
The ability to request custom tetramers for unique peptide antigens and HLA alleles has been instrumental in our epitope mapping studies. Their efficient peptide exchange process has greatly facilitated our high-throughput screening efforts. - M***k. -
Robust Data
The quality of the data we receive from Creative Biolabs is consistently reliable. The comprehensive reports and statistical analysis are invaluable, allowing us to confidently move forward with our research without spending excessive time on data processing. - J***n.
FAQs
How do your assays handle the low affinity of some T-cell receptors?
Our assays are designed to address this challenge. We utilize higher-order multimers, such as dextramers, and advanced staining protocols to enhance binding avidity and prevent TCR internalization, ensuring that even low-affinity T-cell populations are detected.
Do you offer custom solutions for non-classical MHC molecules?
Yes, we specialize in custom solutions. In addition to a vast library of classical MHC I and II alleles, we can develop multimers for non-classical MHC molecules and other non-MHC ligands to meet the unique needs of your research.
Related Services
ELISpot Assay Service
We offer highly sensitive T- and B-cell based ELISpot assays to assess cytokine secretion and T-cell activation at a single-cell level. This is ideal for identifying rare antigen-specific lymphocytes.
Learn More →Immune Cells Function Assay Services
A comprehensive platform to assess immune cell functionality. This includes services for evaluating T cell proliferation, cytokine release, cytotoxicity, and the analysis of immune cell populations through flow cytometry.
Learn More →How to Contact Us
Equipped with advanced technologies, Creative Biolabs is devoted to offering multiple T and B cell-based assays for cancer epitope analysis. We are also experienced in developing epitope-targeted immunotherapies for cancer and autoimmune diseases. If you are interested in learning more about Creative Biolabs' services, please do not hesitate to contact us for more details.
Reference
- Dolton, Garry et al. "Optimized Peptide-MHC Multimer Protocols for Detection and Isolation of Autoimmune T-Cells." Frontiers in immunology vol. 9 1378. 29 Jun. 2018. Distributed under an Open Access license CC BY 4.0, without modification. https://doi.org/10.3389/fimmu.2018.01378
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