ImmunoSEQ is used to analyze TCR repertoire in a high-throughput 96-well plate format, allowing many samples to be evaluated in parallel. For this assay, a genomic DNA library from each sample of interest is used as input. Multiplex PCR primers are used to amplify the CDR3 region of TCR-β.
TCR Identification Service
Creative Biolabs has a new generation of sequencing (NGS) that checks millions of sequences at a time and examines the ability of antigen receptor libraries at very high resolutions. We provide T cell receptor library analysis services including in-depth identification of T cell receptors in human, mouse, rat or rabbit. Based on the TCR sequencing software platform, we will examine and resolve raw T cell receptor sequencing data and provide you with analytical results in tabular and graphical forms.
Introduction What We Can Offer How We Can Help Why Choose Us? Customer Reviews FAQs Related Services Contact Us
Introduction of TCR Identification
Characterization of T cell receptors (TCRs) involved in immune responses is important to the design of tumor immunotherapy and vaccine. The specificity of the interaction between the TCR heterodimer and its peptide-MHC ligand is primarily derived from the highly variable CDR3 regions juxtaposed with the TCR alpha and TCR beta chains, and obtaining the pairing sequences of these regions is the criteria for the functional definition of TCR. Current methods for identifying TCRs in T cell populations are using high throughput single cell sequencing.
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Fig.1 Overview of main strategies to identify relevant TCRs, methods for transduction and options of carrier cells. 1,3
What We Can Offer
Creative Biolabs' deep sequencing of T cell receptor repertoires enables the analysis of TCR status of different individuals at different time points with high resolution. The distribution of the length of the CDR3 gene (digital profile), the CDR relationship, the frequency distribution of the CDR, and the like can be obtained. Intelligent clustering of receptor families can even identify rare TCRs. The V- and J-regions of CDR3α and β chains can be identified through our TCR sequencing analysis software platform.
ImmunoSEQ Assay
Single Cell Immune Profiling
The final products of this assay are the full-length V, D and the J gene segment from each of the input cells for clonotype analysis. Sequencing is performed at a single cell level to obtain paired alpha- and beta-chain information, resulting in high-resolution analysis of the TCR.
How Creative Biolabs' service Can Assist Your Projects
Fig.2 TCR identification. 2,3
Highlights
Native Precision Pairing
We guarantee that every individual receptor chain correctly matches its original biological partner at the single-cell level, ensuring high-fidelity results for all downstream molecular applications.
Unbiased Clone Detection
Our advanced laboratory methods identify rare, high-affinity clones that are typically missed or skewed by traditional amplification techniques, providing a true representation of the repertoire diversity.
Functional Target Prediction
Our specialized analysis predicts antigen targets for unknown receptors, offering a distinct advantage in the discovery, characterization, and molecular engineering of novel immune-based research candidates.
Integrated Synergy
We combine high-capacity analytical platforms with sophisticated structural modeling to offer deep insights that exceed the capabilities of standard industry service providers in this field.
To discuss your project requirements, explore custom validation strategies, and receive a tailored roadmap for your research-grade lead identification - Get a Quote
Customer Reviews
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Unbiased Repertoire Insight
This methodology mitigated stochastic amplification biases frequently observed in prior assays, perhaps enabling a more authentic and rigorous representation of the authentic immune repertoire. - Dr. A**a. J. -
Rapid Functional Validation
Integration of these services enhanced analytical throughput, allowing high-fidelity reporter readouts to refine candidate prioritization and effectively accelerate our laboratory decision-making process. - S**h. W.
FAQs
Why should I choose your discovery platform over standard methods?
Traditional methods often introduce amplification bias, potentially overlooking rare sequences. Our approach ensures an unbiased representation, capturing the full diversity of your cell population.
Can you identify receptors for non-classical targets?
Yes. Our platform is compatible with a diverse array of recognition molecules, including various MHC classes and non-classical targets for research.
Related Services
TCR Generation and Optimization
We perform rigorous functional assays and preclinical animal studies to evaluate the therapeutic potency, persistence, and safety of engineered TCR candidates.
Learn More →scFv Generation and Optimization
We provide specialized engineering to construct high-affinity single-chain variable fragments, enhancing targeting precision for diverse research-grade immune-recognition and diagnostic applications.
Learn More →How to Contact Us
Creative Biolabs provides an end-to-end framework designed to streamline your research from initial samples to validated leads. By combining unbiased discovery methods with comprehensive functional assessment, we eliminate common biases and technical hurdles inherent in traditional immune profiling workflows.
For more details, please feel free to contact us for project quotations and more detailed information.
Reference
- Arnaud, Marion et al. "The Promise of Personalized TCR-Based Cellular Immunotherapy for Cancer Patients." Frontiers in immunology vol. 12 701636. 30 Jul. 2021. https://doi.org/10.3389/fimmu.2021.701636
- Baulu, Estelle et al. "TCR-engineered T cell therapy in solid tumors: State of the art and perspectives." Science advances vol. 9,7 (2023): eadf3700. https://doi.org/10.1126/sciadv.adf3700
- Distributed under an Open Access license CC BY 4.0, without modification.
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