B Cell based Chromatography Assay Service
Creative Biolabs' B cell-based chromatography assay is engineered to deliver a panel of high-quality, target-specific B cells and their corresponding antibody gene sequences with unparalleled speed and efficiency. We provide a clear, direct path from a biological sample to a fully characterized antibody candidate, ready for downstream therapeutic or diagnostic development. This allows you to focus on the science that matters, knowing that your foundational candidates are of the highest quality from day one.
Introduction What We Can Offer How We Can Help Why Creative Biolabs Customer Reviews FAQs Related Services Contact Us
Introduction of Chromatography Assay
Affinity-based chromatography is a powerful and specific separation method used for bioactive compound isolation and purification employing the binding affinity between immune complexes. Specific monoclonal antibodies or patient immunoglobulin have been used to capture human or mouse tumor tissue-derived antigens, HLA-restricted tumor-specific antigens, and neoantigens. Following the capture process, the proteins bound to the chromatography are eluted and evaluated by various other methods.
To gain a comprehensive understanding of how our services can be applied to your specific research and development needs - Request a consultation.
Fig.1 Immunoaffinity chromatography for protein purification.
What We Can Offer
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Antibody Development and Purification
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Source of Antibodies
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Antibodies Purification Using Chromatography
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Antigen immunized animals
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Infected patients
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Cloning and expression in cells
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Protein A/G: purify IgG antibodies from the positive samples.
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Ni-NTA: isolate recombinant antibodies with His tag.
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Immunoaffinity Chromatography Column: use a known antigen protein to perform affinity purification.
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Fig.2 Different formats of antibodies.1,3
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Antigen Protein Purification
Antigen protein could be purified from two pathways: immunoaffinity chromatography and receptor-ligand-based affinity chromatography assay.
Chromatography Assay Services at Creative Biolabs
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Immunoaffinity Chromatography
High-specificity monoclonal antibodies are used to purify antigens based on binding affinity.
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Support Materials
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Antibody Conjugation Methods
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Elution Conditions
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Low-pressure chromatography: resin or polymers like polyacrylamide and polymethacrylate.
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High-performance liquid chromatography (HPLC).
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Via antibody's amine or carboxyl.
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Via exposed free sulfhydryl group on Fab.
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Via carbohydrate residues on the Fc region.
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Via intermedia, such as Biotin-avidin affinity, protein A, or G.
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Optimize loading buffer by modifying pH and concentration of the solution.
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Optimize elution buffer by adjusting pH, the composition of the solution, and using chelating agents.
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Receptor-ligand-based Affinity Chromatography
Biotinylated monoclonal antibodies are incubated with the antigen protein mixture, and the formed antigen-antibody complex is purified by an avidin column. Finally, the bound antigens are separated using a proper elution buffer.
Fig.3 Specific antigen-antibody complex purification using chromatography.2,3
Highlights
High Specificity
Our chromatography-based method enables the precise isolation of B cells with the highest affinity for your target antigen. This ensures that you obtain the most specific and potent antibody candidates from your initial sample.
Large-scale
The assay platform is built to handle a large volume of B cells from a single sample. This allows for the rapid screening of a vast antibody repertoire, increasing the probability of discovering rare, high-value clones.
High Efficiency
By directly isolating antigen-specific B cells, our streamlined workflow bypasses time-consuming and inefficient steps like hybridoma fusion, dramatically accelerating the timeline from sample to gene sequence.
Cost-effective Price
Our efficient, high-throughput process significantly reduces labor and material costs compared to traditional methods. This allows you to achieve a higher success rate with a lower overall investment.
Discover how a partnership with us can streamline your research - Get a quote today.
Customer Reviews
Exceptional Speed and Efficiency
Using Creative Biolabs' B cell-based chromatography assay in our research has significantly improved the speed of our lead antibody identification. The process is remarkably streamlined, saving us months of development time. - Da Rs.
Confidence in Candidate Quality
The assay provided highly specific B cells, eliminating months of frustrating post-screening work and giving us confidence in our candidates from day one. We received a panel of clones that were ready for immediate downstream work. - Ae Mn.
FAQs
What are the key advantages of this method for discovering antibodies from immunized animals?
The primary advantage is speed and specificity. Our assay significantly compresses the timeline from immunization to candidate identification. By directly selecting for antigen-specific B cells, we ensure that the candidates you receive have already undergone natural affinity maturation.
Can this assay be used to identify antibody fragments or scFv?
Yes. We can isolate B cells that produce a variety of antibody formats, including fragments. As demonstrated in published research, specialized affinity ligands such as Protein L can be effectively used for purifying these fragments, and our upstream assay is perfectly compatible with these downstream purification protocols.
Related Services
Amino Acid Sequencing Service
Our liquid chromatography and mass spectrometry assays provide precise amino acid sequencing to verify the identity and integrity of your protein candidates.
Antibody Purity Testing
We perform immunoprecipitation assays and SDS-PAGE to rigorously test and confirm the purity of your antibody samples before further development.
How to Contact Us
Creative Biolabs' B cell-based chromatography assay is a transformative solution for the challenges of modern antibody discovery. We deliver the speed, specificity, and cost-effectiveness you need to succeed in a competitive landscape. Our commitment to innovation and deep scientific expertise ensures that your project is in the hands of a trusted partner. Please contact us and consult the service of your interest.
References
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De Groof, Timo W M et al. "Nanobodies: New avenues for imaging, stabilizing and modulating GPCRs." Molecular and cellular endocrinology vol. 484 (2019): 15-24. https://doi.org/10.1016/j.mce.2019.01.021
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Richards, Jessie et al. "Novel methods of immunogenic antigen selection for serological diagnosis of Parelaphostrongylus tenuis infection." Scientific reports vol. 13,1 10989. 7 Jul. 2023. https://doi.org/10.1038/s41598-023-37481-7
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Distributed under an Open Access license CC BY 4.0, without modification.
For Research Use Only | Not For Clinical Use
