B Cell based Quenching Assay Service
Creative Biolabs provides a direct solution to the primary bottlenecks in antibody discovery. Our core capability is the ability to screen millions of single B cells for functional antibodies, ensuring that only the most promising candidates are advanced. You can expect to receive comprehensive data on antibody specificity and functionality, and a clear path to sequencing. Our platform can assist in projects targeting a wide range of antigens, including membrane proteins, viral antigens, and complex multi-protein complexes.
Introduction What We Can Offer Workflow Why Creative Biolabs Customer Reviews FAQs Related Services Contact Us
Summary of Quenching Assay
An appropriate method that can be used to monitor the immune responses between the antibody and antigen peptides is valuable for immunogenic epitope discovery. Creative Biolabs offers a bead-based immunoassay to identify epitopes that can bind to specific antibodies. The candidate peptides are conjugated to beads and incubated with positive antibodies. Fluorescent-labeled anti-antibody and different lasers combined to detect the Ab: Ag complex formed on the beads.
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Fig.1 Graphene oxide-based fluorescence quenching for the detection of IgG proteins.1
What We Can Offer
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Complementary Validation Assays
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Complementary Assays
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Detailed Description
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Dose-dependent Ab: Ag Reactive
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Immunized plasma samples are diluted to multiple doses to monitor dose-related reactions to epitope peptides.
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Peptide-specific ELISA
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The specific antibody absorption response to each epitope is confirmed using ELISA.
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Peptide-induced T-cell Responses
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Peptides are expressed on cell lines and inoculated with immunized PBMC to test IFN-γ production, cytotoxicity, and a competitive antibody-based inhibition test.
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B Cell-based Quenching Assay at Creative Biolabs
Creative Biolabs provides a set of beads that is coded with unique labels and has a discriminative spectrum from each other, and tailored cancer epitope analysis services using this convenient and moderate-throughput bead-based assay.
Key Features of the Service
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Customer Reviews
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Unique Application
The B cell-based quenching assay facilitated our work on a complex membrane protein. The ability to screen for an internalization-based quenching event was a huge advantage, enabling us to discover antibodies that were missed by standard binding assays. - J***e A.
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Tangible Benefits
We were able to reduce our lead candidate identification timeline by several months with Creative Biolabs' service. The detailed data report and high-quality sequences we received were invaluable and helped streamline our downstream processes. - R***t L.
FAQs
What types of antigens can be used with this assay?
Our platform is highly versatile and can accommodate a wide range of antigens, including recombinant proteins, cell lines, and viral antigens. We can discuss the specific requirements for your target during an initial consultation.
How does the B cell-based quenching assay compare to traditional methods like ELISA or FACS?
While ELISA and FACS are powerful tools, they typically screen for binding affinity, often requiring multiple, sequential steps to determine functionality. Our quenching assay screens for a direct functional event (antigen internalization), collapsing multiple steps into a single, high-throughput workflow. This saves significant time and resources.
Related Services
T and B Cell-based ELISA Assay
A traditional but highly reliable method for quantifying antibody levels or T-cell activation, complementing our high-throughput screens with a robust, quantitative readout.
B Cell-based Microarray Assay
This service allows for the simultaneous screening against a diverse panel of antigens, providing a broad profile of your antibody candidates.
B Cell-based Flow Cytometry Assay
Perfect for in-depth, single-cell analysis of B cell populations, enabling detailed phenotyping and sorting based on surface marker expression and antigen-binding characteristics.
How to Contact Us
At Creative Biolabs, you just need to tell us what target antigen you are interested in. Our experienced in-house experts will provide comprehensive solutions to investigate immunogenic epitopes in the antigen and discover novel candidate epitopes. Please contact us and get the protocol for the quenching assay.
Reference
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Huang, Ao et al. "Quantitative Fluorescence Quenching on Antibody-conjugated Graphene Oxide as a Platform for Protein Sensing." Scientific reports vol. 7 40772. 13 Jan. 2017. Distributed under an Open Access license CC BY 4.0, without modification. https://doi.org/10.1038/srep40772
For Research Use Only | Not For Clinical Use
