T Cell based High Throughput Multiplexed Assay Service

Creative Biolabs is expertise in the mechanism of T cell antigen recognition and is experienced in applying immunological technologies for antigen discovery. Now, we provide high-throughput multiplexed assays to identify T-cell epitopes for basic and translational research, such as drugs and vaccine design for infectious diseases.

Current High-throughput Multiplexed Approaches

Multiple approaches are utilized to develop a high-throughput multiplexed method to explore a large scale of potential epitopes in the antigen protein. Most of the approaches display the peptide-MHC complex on cells, such as tumor cells, yeast, or baculovirus, and co-culture with cognate T cells or use soluble TCRs to assess T cell binding and activation. Recently, a team delivered the genome-wide antigen to cells and processed it by endogenous MHC-I molecules to imitate the natural antigen-presented pathway and use the granzyme B activity as the effector molecule to monitor T-cell responses.

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Fig.1 Discovery of T cell antigens by high-throughput screening of synthetic minigene libraries.¹

What We Can Offer

High Throughput Multiplexed Assay Services at Creative Biolabs Services

At Creative Biolabs, we provide a systematic high-throughput antigen genome screening assay to identify cancer epitopes associated with T cell immunity, providing potential targets for diagnostics, antibody-mediated therapy, and vaccine development.

Workflow

• Cancer Antigen Proteome Library Construction

A method that comprehensively profiles cancer antigens is crucial for identifying T cell epitopes that are physiologically meaningful in vivo. We have developed a high-throughput technology to construct an ORF library covering the entire coding domain of cancer antigens. In detail, each OFR is cloned into our vector and expressed using in vitro transcription/translation kit.

• Polyclonal T-cell Stimulation

PBMCs are stimulated with full-length antigens to produce enough antigen-specific T cells. The specific T cells are cultured to more than 10 billion, and CD4+, CD8+ T cells are sorted for the following screening.

• Epitope Screening

Expanded CD4+ T cells are co-cultured with the synthesized antigen protein library and cognate antigen-presenting cells (APC).

Expanded CD8+ T cells are mixed with antigen proteins presented by artificial APCs that are generated from HLA cDNA and peptides co-transfected COS-7 cells.

• Monitor T Cell Immune Response

A read-out assay, such as a ³H-thymidine incorporation assay or an ELISpot assay for cytokine secretion, is adopted to screen the antigen-specific responses.

Fig.2 Screening results of IFNγ-release ELISpot.¹

Highlights

Scientific Expertise and Precision

Our team comprises experienced immunologists and bioinformaticians who are dedicated to delivering accurate, high-quality data. We use state-of-the-art bead-based and microfluidic technology to minimize technical bias and ensure the reproducibility of your results.

Unmatched Throughput and Efficiency

Our proprietary platform allows for the rapid, simultaneous quantification of dozens of biomarkers from a single, minute sample volume. This high-throughput capability drastically reduces your experimental time and resource consumption, accelerating your research timelines.

Comprehensive and Actionable Data

Unlike single-plex assays, our multiplexed platform provides a rich, holistic view of the T-cell response. We deliver comprehensive data reports and a final scientific summary, complete with expert insights and recommendations to advance your project.

Dedicated Partnership and Customization

Our team works closely with you to understand your unique project needs, offering fully customized assay panels and experimental designs. From initial consultation to final data delivery, we are your trusted partner in discovery.

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Customer Reviews

• Improved efficiency
  Using Creative Biolabs' assay in our research has significantly improved the efficiency of our epitope discovery program. We were able to screen thousands of candidates that would have taken us a year to complete with our conventional methods. – Dr. S*, Chief Scientific Officer.
• Comprehensive data
  The ability to simultaneously measure dozens of analytes from a single sample has given us a level of detail we've never had before. This comprehensive data set has been invaluable in understanding the complex nuances of T-cell activation. – A*. L***, Principal Investigator.

FAQs

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How to Contact Us

Our T cell-based high-throughput multiplexed assay helps you accelerate your immunotherapy research by providing comprehensive, high-resolution data at an unprecedented scale through our advanced multiplexing and automation technologies.

Contact us today to discuss your project needs and discover how our T cell-based high-throughput multiplexed assay can help you achieve your goals faster.

Reference

1. Hondowicz, Brian D et al. "Discovery of T cell antigens by high-throughput screening of synthetic minigene libraries." PloS one vol. 7,1 (2012): e29949. Distributed under an Open Access license CC BY 4.0, without modification. https://doi.org/10.1371/journal.pone.0029949

For Research Use Only | Not For Clinical Use