We provide fully customized services tailored to your specific project needs. Our experts will work with you to optimize assay design, select relevant analytes, and ensure the workflow aligns perfectly with your research goals.
T Cell based High Throughput Multiplexed Assay Service
Creative Biolabs is expertise in the mechanism of T cell antigen recognition and is experienced in applying immunological technologies for antigen discovery. Now, we provide high-throughput multiplexed assays to identify T-cell epitopes for basic and translational research, such as drugs and vaccine design for infectious diseases.
Introduction What We Can Offer How We Can Help Why Creative Biolabs Customer Reviews FAQs Related Services Contact Us
Current High-throughput Multiplexed Approaches
Multiple approaches are utilized to develop a high-throughput multiplexed method to explore a large scale of potential epitopes in the antigen protein. Most of the approaches display the peptide-MHC complex on cells, such as tumor cells, yeast, or baculovirus, and co-culture with cognate T cells or use soluble TCRs to assess T cell binding and activation. Recently, a team delivered the genome-wide antigen to cells and processed it by endogenous MHC-I molecules to imitate the natural antigen-presented pathway and use the granzyme B activity as the effector molecule to monitor T-cell responses.
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Fig.1 Discovery of T cell antigens by high-throughput screening of synthetic minigene libraries.1
What We Can Offer
Customized Service
Unmatched Scale and Efficiency
Our high-throughput platform is designed to handle the most demanding projects. We can screen thousands of samples and measure dozens of analytes in a fraction of the time required by conventional methods, ensuring you get the data you need faster.
Available Cytokines and Chemokines Analysis
We provide the following popular cytokine and chemokine release measurement services to monitor T cell responses.
| Multiple Analytes Available | |||||||
|---|---|---|---|---|---|---|---|
| IL-1b | IL-2 | IL-3 | IL-4 | IL-5 | IL-6 | IL-8 | IL-10 |
| IL-12 | IL-13 | IL-17 | IL-17A | IL-22 | IL-23 | IFNγ | IFNβ |
| TNF | TNFα | GM-CSF | TGFβ | CXCL10/IP-10 | CCL4/MIP-1b | CXCL9/MIG | CCL3/MIP-1a |
| TNFβ | |||||||
High Throughput Multiplexed Assay Services at Creative Biolabs Services
At Creative Biolabs, we provide a systematic high-throughput antigen genome screening assay to identify cancer epitopes associated with T cell immunity, providing potential targets for diagnostics, antibody-mediated therapy, and vaccine development.
Workflow
- Cancer Antigen Proteome Library Construction
A method that comprehensively profiles cancer antigens is crucial for identifying T cell epitopes that are physiologically meaningful in vivo. We have developed a high-throughput technology to construct an ORF library covering the entire coding domain of cancer antigens. In detail, each OFR is cloned into our vector and expressed using in vitro transcription/translation kit.
- Polyclonal T-cell Stimulation
PBMCs are stimulated with full-length antigens to produce enough antigen-specific T cells. The specific T cells are cultured to more than 10 billion, and CD4+, CD8+ T cells are sorted for the following screening.
- Epitope Screening
Expanded CD4+ T cells are co-cultured with the synthesized antigen protein library and cognate antigen-presenting cells (APC).
Expanded CD8+ T cells are mixed with antigen proteins presented by artificial APCs that are generated from HLA cDNA and peptides co-transfected COS-7 cells.
- Monitor T Cell Immune Response
A read-out assay, such as a 3H-thymidine incorporation assay or an ELISpot assay for cytokine secretion, is adopted to screen the antigen-specific responses.
Fig.2 Screening results of IFNγ-release ELISpot.1
Highlights
Scientific Expertise and Precision
Our team comprises experienced immunologists and bioinformaticians who are dedicated to delivering accurate, high-quality data. We use state-of-the-art bead-based and microfluidic technology to minimize technical bias and ensure the reproducibility of your results.
Unmatched Throughput and Efficiency
Our proprietary platform allows for the rapid, simultaneous quantification of dozens of biomarkers from a single, minute sample volume. This high-throughput capability drastically reduces your experimental time and resource consumption, accelerating your research timelines.
Comprehensive and Actionable Data
Unlike single-plex assays, our multiplexed platform provides a rich, holistic view of the T-cell response. We deliver comprehensive data reports and a final scientific summary, complete with expert insights and recommendations to advance your project.
Dedicated Partnership and Customization
Our team works closely with you to understand your unique project needs, offering fully customized assay panels and experimental designs. From initial consultation to final data delivery, we are your trusted partner in discovery.
Discover how Creative Biolabs' T cell-based high-throughput multiplexed assay can streamline your research - Get a quote today.
Customer Reviews
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Improved efficiency
Using Creative Biolabs' assay in our research has significantly improved the efficiency of our epitope discovery program. We were able to screen thousands of candidates that would have taken us a year to complete with our conventional methods. – Dr. S*, Chief Scientific Officer. -
Comprehensive data
The ability to simultaneously measure dozens of analytes from a single sample has given us a level of detail we've never had before. This comprehensive data set has been invaluable in understanding the complex nuances of T-cell activation. – A*. L***, Principal Investigator.
FAQs
What is the minimum sample volume required for the assay?
One of the key benefits of our technology is its low sample volume requirement. It can quantify dozens of biomarkers from a single, minute sample, making it ideal for studies with limited clinical material.
What measures are in place to ensure data quality and minimize technical bias?
Our assay incorporates advanced bead-based and microfluidic technology designed to ensure high data quality and reproducibility. We have robust quality control checks at every stage of the workflow, and our experienced team carefully reviews all data to ensure accuracy.
Related Services
T and B Cell-based ELISA Assay
Our platform utilizes high-throughput plates to analyze multiple samples and screen a panel of peptides for immunodominant epitopes, providing accurate and reliable data.
Learn More →T Cell-based Cytometric Bead Array Assay
A powerful technique that combines ELISA with flow cytometry to simultaneously test multiple analytes. This assay is highly sensitive, allowing for the analysis of up to 30 different proteins from a small sample volume.
Learn More →How to Contact Us
Our T cell-based high-throughput multiplexed assay helps you accelerate your immunotherapy research by providing comprehensive, high-resolution data at an unprecedented scale through our advanced multiplexing and automation technologies.
Contact us today to discuss your project needs and discover how our T cell-based high-throughput multiplexed assay can help you achieve your goals faster.
Reference
- Hondowicz, Brian D et al. "Discovery of T cell antigens by high-throughput screening of synthetic minigene libraries." PloS one vol. 7,1 (2012): e29949. Distributed under an Open Access license CC BY 4.0, without modification. https://doi.org/10.1371/journal.pone.0029949
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