P450-Metric™ Cytochrome P450 3A4 (CYP3A4) ELISA Kit

CAT#: ITS-0326-YL7
Product Type: Assay Kit
Species: Human, Mouse
Brand: P450-Metric™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Quantifies CYP3A4 using a double-antibody sandwich ELISA.
Brand
P450-Metric™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
This state-of-the-art double-antibody sandwich ELISA kit ensures the highly accurate quantitative measurement of Cytochrome P450 3A4, a predominant oxidative enzyme, in biological samples. Boasting outstanding analytical specificity, this research-use-only platform enables researchers to efficiently assess significant systemic drug clearance pathways. It is absolutely crucial for predicting severe drug-drug interactions and establishing reliable metabolic profiles during high-throughput preclinical pharmacokinetic and comprehensive ADME research.
Features
Optimized for high-throughput preclinical screening, this assay provides highly accurate quantification of CYP3A4, the most critical enzyme for evaluating systemic drug clearance and metabolic liabilities.
Process Relevance
Quantifying CYP3A4 to establish systemic clearance rates and evaluate multi-pathway drug-drug interactions.
Application Stage
Preclinical drug discovery, ADME profiling, and pharmacokinetic research
Applications
This sandwich ELISA accurately quantifies target enzyme concentrations in cell lysates, tissue homogenates, and other biological fluids.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates
Research Areas
ADME; Drug Metabolism; Pharmacokinetics; Toxicology
Size
48 / 96 tests
Data Output Type
Absorbance (OD)
LOD
Mouse: 0.57 ng/mL
Human: 0.121 ng/mL
Species
Human, Mouse
Storage
Upon receipt, please follow the storage recommendations in the USER MANUAL to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. Do not store at elevated temperatures for extended periods.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Cytochrome P450 3A4 is the most abundant hepatic metabolic enzyme, responsible for the biotransformation of over half of all currently marketed pharmaceuticals. Consequently, establishing its interaction profile is the cornerstone of ADME research for predicting metabolic clearance and severe drug-drug interactions.
For Research Use Only | Not For Clinical Use
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