GlycoErase™ HEK293T MGAT4B Knockout Cell Line (CAT#: GLJF-0425-JF447)

Description	We are pleased to introduce an MGAT4B knockout HEK293T cell line, specifically designed to aid in the study of N-glycan processing and glycoprotein function. This cell line lacks the MGAT4B-encoded mannosyl (alpha-1,3-)-glycoprotein beta-1,4-O-acetylglucosaminyltransferase 4B, an enzyme that participates in the synthesis of complex N-linked oligosaccharides. Researchers can utilize this model to explore the impact of disrupting MGAT4B function on N-glycan processing and glycoprotein function.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293T
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Penicillin/Streptomycin
Application	Functional assay
Size	2 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	MGAT4B
Full Name	Alpha-1,3-mannosyl-glycoprotein 4-Beta-O-acetylglucosaminyltransferase B
Alternative Name	GnT-Ivb
Location	5q35.3
Gene ID	11282
Summary	Regulates the formation of tri- and multiantennary branching structures in the Golgi apparatus by catalyzing the transfer of GlcNAc in a beta-1,4 linkage.