Glycomics Quantitative Analysis Service in Food

Glycomics Quantitative Analysis Service in Food: Precision Nutrition, Molecularly Verified.

Food glycomics quantitative analysis services are an interdisciplinary technology focused on systematically analyzing the structure, functions, and content of carbohydrate compounds in foods. Unlike Milk Glycomics, which primarily focuses on a single food matrix, food glycomics covers a broader range, encompassing diverse matrices such as grains, fruits, honey, and processed foods. Its complexity stems from the diverse structure of carbohydrates (e.g., monosaccharides, oligosaccharides, polysaccharides, and glycoconjugates) and their dynamic changes during food processing. With the increasing demand for precision nutrition and food safety, Glycomics Analysis has become a core tool for food traceability, functional ingredient development, and quality control. Creative Biolabs' glycomics quantitative analysis service in food helps you unlock product differentiation and ensure quality assurance through advanced bottom-up chemical digestion and high-resolution mass spectrometry platforms.

Fig.1 Glycomics analysis workflow for meat samples.^1,3

How Can We Assist Your Project?

Creative Biolabs' service is designed to bridge the data gap between bulk nutritional information and specific molecular functions. By precisely quantifying individual polysaccharide types, we provide the foundational data necessary for R&D breakthroughs in nutrition, agriculture, and quality assurance.

• Precision prebiotic design: We move beyond labeling a substance as "fiber" to quantify its specific components (e.g., arabinan, β-glucan, xylan). This allows clients to scientifically select ingredients that optimally feed targeted beneficial gut bacteria (such as Bifidobacteria) for condition-specific functional foods.
• Product authentication: Our high-resolution fingerprinting provides molecular proof of source and purity, essential for authentication associated with high-value ingredients, such as specific botanical extracts or specialized cereal fibers.
• Agricultural R&D: By quantifying the cell wall polysaccharides (e.g., cellulose, xyloglucan) in crop varieties, we provide data critical for precision breeding programs focused on improving crop resistance to environmental stress.

We utilize a high-throughput, standardized process, centered around the multiplexed, quantitative bottom-up glycomics approach, ensuring rapid and reliable data delivery.

Technical Challenges and Solutions

The technical complexity of food glycomics is exceptionally high due to:

• Structural diversity and complexity: Polysaccharides are highly diverse, featuring multiple monosaccharides, various anomeric configurations, and diverse glycosidic linkages. Our proprietary chemical depolymerization method is designed to be broadly reactive, breaking down a wide range of common polysaccharide linkages simultaneously, enabling multiplexing that single-enzyme assays cannot.
• Lack of high-throughput methods: Conventional analysis involves complex, laborious extraction and fractionation steps, followed by specific monosaccharide or linkage analyses, making it impractical for large-scale R&D. We optimize the entire workflow (from homogenization to solid-phase extraction (SPE) cleanup) into a multiplexed high-throughput plate system and implement automated Glycoinformatics for rapid data processing.
• Matrix effects: The food matrix (lipids, proteins, salts) can suppress ionization during mass spectrometry. We perform rigorous two-stage SPE cleanup in a high-throughput plate format to ensure sample cleanliness and minimize matrix interference.

Why Choose Us?

Choosing Creative Biolabs means selecting a partner that provides scientifically rigorous, high-throughput data essential for verifying product functions and integrity in the complex food matrix. Our unique advantages eliminate the bottlenecks inherent in older analytical methods.

Published Data

This publication introduces a novel quantitative glycomics method for analyzing the polysaccharide content in food matrices using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The core of the approach, as visually represented in the following figure, involves several key steps for the qualitative identification and absolute quantitation of polysaccharides. Initially, the polysaccharides in the food sample are depolymerized into smaller, characteristic oligosaccharide fragments using a nonenzymatic chemical reaction dubbed Fenton's initiation towards defined oligosaccharide groups (FITDOG). These resulting oligosaccharides, which serve as a unique "fingerprint" for their parent polysaccharide, are then separated and analyzed using high-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry (HPLC-QTOF-MS). The chromatographic separation helps to distinguish the oligosaccharides, and the tandem mass spectrometry (MS/MS) provides their compositional identity. Quantitation is achieved by measuring the peak areas of the most abundant, representative oligosaccharides and converting these values into absolute concentrations using external calibration curves derived from pure polysaccharide standards. This comprehensive workflow enables the simultaneous, multiplexed measurement of up to nine common food polysaccharides, including starch, cellulose, and various non-starch polysaccharides, with demonstrated accuracy and reproducibility.

Fig.2 Quantitative analysis of polysaccharides using FITDOG.^2,3

FAQs

Customer Review

Extended Services

• N-Glycan and O-Glycan Glycoproteomic Analysis services: Advanced analytical techniques are used to analyze N- and O-glycans in different samples.
• Metabolomics service: Quantify the crucial downstream functional products of polysaccharide fermentation, such as short-chain, long-chain fatty acids and other bioactive small molecules, complementing your glycomics data by validating function.
• Proteomics service: Analyze the protein and enzyme content of your raw material or the expression profile of the gut microbiota, providing insight into the protein-glycan interactions influencing digestibility and health outcomes.
• Bioinformatics & Glycoinformatics: Our dedicated data science team specializes in complex statistical analysis, including principal component analysis (PCA) for authentication and machine learning for structure-function mapping to turn raw data into strategic insights.

How to Contact Us

Creative Biolabs stands as your strategic partner in the future of food science. We transform the challenge of complex carbohydrate analysis into a competitive advantage, providing the high-resolution, quantitative data necessary to validate functional claims, ensure product authenticity, and accelerate precision R&D. Please reach out to our expert team today to discuss your specific project requirements, sample complexity, and desired turnaround time.

References

1. Chia, Sean, et al. "An integrative glycomic approach for quantitative meat species profiling." Foods 11.13 (2022): 1952. https://doi.org/10.3390/foods11131952.
2. Bacalzo Jr, Nikita P., et al. "Quantitative bottom-up glycomic analysis of polysaccharides in food matrices using liquid chromatography-tandem mass spectrometry." Analytical Chemistry 95.2 (2022): 1008-1015. https://doi.org/10.1021/acs.analchem.2c03707.
3. Distributed under an Open Access license CC BY 4.0, without modification.

Related Services

• N-Glycan-based Glycoproteomic Quantitative Analysis
• O-Glycan-based Glycoproteomic Quantitative Analysis
• Proteomic Quantitative Analysis
• Glycomics Quantitative Analysis in Milk