GlycoErase™ HEK293T MGAT5 Knockout Cell Line (CAT#: GLJF-0425-JF450)

Description	For researchers exploring N-linked oligosaccharide synthesis, we are pleased to offer an MGAT5 knockout HEK293T cell line. This model lacks the MGAT5-encoded alpha-1,6-mannosyl-glycoprotein 6-beta-O-acetylglucosaminyltransferase, an enzyme that catalyzes the addition of beta-1,6-N-acetylglucosamine to the alpha-linked mannose of biantennary N-linked oligosaccharides. By disrupting MGAT5 function, this cell line provides a valuable tool for understanding the consequences of impaired N-linked oligosaccharide synthesis and its impact on cellular processes.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293T
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Penicillin/Streptomycin
Application	Functional assay
Size	2 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	MGAT5
Full Name	Alpha-1,6-mannosylglycoprotein 6-Beta-O-acetylglucosaminyltransferase
Alternative Name	GNT-V, MGAT5A
Location	2q21.2-q21.3
Gene ID	4249
Summary	Catalyzes the addition of beta-1,6-N-acetylglucosamine to biantennary N-linked oligosaccharides, influencing cell adhesion and migration.