GlycoErase™ MGAT1 Knockout A549 Cell Line (CAT#: GLJF-0425-JF571)

Description	MGAT1 knockout A549 cell line lacks MGAT1 protein, alpha-1,3-mannosyl-glycoprotein 2-beta-O-acetylglucosaminyltransferase, involved in oligosaccharide synthesis. MGAT1 knockout in A549 cells enables study of Oligosaccharide synthesis disruption in lung cancer, potentially broadly affecting N-glycan branching, protein glycosylation and diverse cellular processes dependent on complex N-glycan structures.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:3~1:6
Cell Line	A549
Primary Disease	Non-small cell lung cancer
Lineage	Lung
Lineage Subtype	Non-small cell lung cancer

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	F-12K & FBS & Penicillin/Streptomycin
Application	Functional assay
Size	2 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	MGAT1
Full Name	Alpha-1,3-mannosyl-glycoprotein 2-Beta-O-acetylglucosaminyltransferase
Alternative Name	MGAT, GnTI, GLCNAC-TI, GLYT1, GNT-1
Location	5q35.3
Gene ID	4245
Summary	Medial-Golgi enzyme essential for the synthesis of hybrid and complex N-glycans, contributing to glycan diversity and function.