GlycoErase™ PRKAG2 Knockout HepG2 Cell Line (CAT#: GLJF-0825-JF708)

Description	GlycoErase™ PRKAG2 knockout HepG2 cell line removes PRKAG2, an AMPK γ subunit involved in nucleotide sensing. Mutations in PRKAG2 lead to glycogen storage cardiomyopathy and Wolff-Parkinson-White syndrome. This knockout model enables mechanistic studies of nucleotide binding, metabolic regulation, and glycogen metabolism in liver cells.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:4~1:6
Cell Line	HepG2
Primary Disease	Hepatoblastoma
Lineage	Liver
Lineage Subtype	Hepatoblastoma

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Glutamine
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	PRKAG2
Full Name	Protein Kinase Amp-Activated Non-Catalytic Subunit Gamma 2
Alternative Name	AAKG; CMH6; WPWS; AAKG2; H91620p
Location	7q36.1
Gene ID	51422
Summary	This gene encodes an AMPK gamma subunit that senses cellular energy levels and inhibits lipid and cholesterol synthesis enzymes when activated. Mutations cause cardiac-related syndromes and glycogen storage disease.