GlycoErase™ PTGER4 Knockout HEK293 Cell Line (CAT#: GLJF-0825-JF477)

Description	GlycoErase™ PTGER4 knockout HEK293 cell line is a HEK293 model without PTGER4 expression. PTGER4 encodes a prostaglandin E2 (PGE2) receptor, a GPCR that activates TCF signaling, regulates COX-2 mRNA stability, and promotes phosphorylation of GSK3. The knockout enables exploration of prostaglandin-driven immune responses, inflammation, and tumor signaling in human cells, supporting applications in immunology, oncology, and inflammation-targeted therapies.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	FBS & Penicillin/Streptomycin & Proline & RPMI
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	PTGER4
Full Name	Prostaglandin E Receptor 4
Alternative Name	EP4; EP4R
Location	5p13.1
Gene ID	5734
Summary	The protein encoded by this gene belongs to the G-protein coupled receptor family and is one of four receptors for prostaglandin E2 (PGE2). It can activate T-cell factor signaling, mediate PGE2-induced expression of early growth response 1 (EGR1), modulate cyclooxygenase-2 mRNA stability, and promote phosphorylation of glycogen synthase kinase-3. Mouse knockout studies suggest roles in neonatal cardiovascular adaptation, osteoporosis, and initiating skin immune responses.