GlycoErase™ TPI1 Knockout HEK293 Cell Line (CAT#: GLJF-0825-JF486)

Description	GlycoErase™ TPI1 knockout HEK293 cell line is a HEK293 model without TPI1 expression. TPI1 encodes triosephosphate isomerase, catalyzing the reversible interconversion of glyceraldehyde-3-phosphate and dihydroxyacetone phosphate in glycolysis and gluconeogenesis. Deficiency causes TPI deficiency, a rare metabolic disorder. This knockout provides a human cellular model to investigate glycolytic control, redox and energy balance, and rare enzymopathies, supporting metabolic and disease-focused research.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	FBS & Penicillin/Streptomycin & Proline & RPMI
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	TPI1
Full Name	Triosephosphate Isomerase 1
Alternative Name	TIM; TPI; TPID; HEL-S-49
Location	12p13.31
Gene ID	7167
Summary	This gene produces an enzyme that catalyzes the reversible interconversion of glyceraldehyde 3-phosphate and dihydroxyacetone phosphate in glycolysis and gluconeogenesis. It is a homodimer, and mutations lead to triosephosphate isomerase deficiency. Pseudogenes occur on chromosomes 1, 4, 6, and 7, and alternative splicing yields multiple variants.