GlycoErase™ XXYLT1 Knockout HepG2 Cell Line (CAT#: GLJF-0425-JF1131)

Description	XXYLT1 knockout HepG2 cell line lacks XXYLT1 protein, with xylosyl alpha 1,3-xylosyltransferase activity, involved in O-glycan processing in the ER membrane of liver cells. XXYLT1 knockout in HepG2 cells enables study of O-glycan processing disruption in liver cancer ER, potentially affecting ER-localized O-glycan synthesis in hepatocytes and O-glycan dependent protein functions within the ER and broader liver tumor context.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:4~1:6
Cell Line	HepG2
Primary Disease	Hepatoblastoma
Lineage	Liver
Lineage Subtype	Hepatoblastoma

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Glutamine
Application	Functional assay
Size	2 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	XXYLT1
Full Name	Xyloside xylosyltransferase 1
Alternative Name	FLJ35155, C3orf21
Location	3q29
Gene ID	152002
Summary	Xylosyltransferase located in the endoplasmic reticulum membrane; involved in O-glycan processing.