GlycoFlux™ Human PHKG2 Overexpression HEK293T Cell Line (CAT#: GLJF-0925-JF555)

Description	GlycoFlux™ human PHKG2 overexpression HEK293T cell line is a stable glycoengineered line that constitutively overexpresses the hepatic γ2 catalytic subunit of phosphorylase kinase. It enables liver-style glycogenolytic regulation to study glycan remodeling, bile-acid receptor glycosylation, and secretion under nutrient shift paradigms.
Product Type	Overexpression Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293T
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	qPCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Penicillin/Streptomycin
Application	Mechanistic studies; Exploration of glycosylation and signaling pathways in cancer, metabolic, and immune-related diseases; Drug target validation and other functional assays.
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	PHKG2
Full Name	Phosphorylase Kinase Catalytic Subunit Gamma 2
Alternative Name	GSD9C
Location	16p11.2
Gene ID	5261
Summary	The hepatic gamma isoform of phosphorylase kinase is encoded by this gene, part of a 16-subunit complex. The gamma subunit contains the active site, with alpha and beta serving regulatory functions. Defects cause glycogen storage disease type 9C (autosomal liver glycogenosis). Several splice isoforms have been described.