Quantitative Human 5HTR1A (Glycosylated) Sandwich ELISA Kit

The quantitative human 5HTR1A (glycosylated) sandwich ELISA kit is designed to detect human 5-hydroxytryptamine receptor 1A (5HTR1A) levels. 5HTR1A is a G protein-coupled receptor that binds serotonin, playing a crucial role in regulating mood, anxiety, and various physiological processes in the central nervous system. It is a target for numerous antidepressant and anxiolytic drugs. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.092 ng/mL, which can accurately detect low concentrations of 5HTR1A in the sample.

5HTR1A

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates

100 μL

0.092 ng/mL

Quantitative sandwich ELISA

1 ng/mL-10 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

5-Hydroxytryptamine receptor 1A

5HTR1A; 5-Hydroxytryptamine receptor 1A; 5HTR1-A; Serotonin receptor 1A

10, 11, 24

The quantitative human 5HTR1A (glycosylated) sandwich ELISA kit is valuable in research investigating psychiatric disorders such as depression and anxiety, as well as studies on the effects of serotonergic drugs. Researchers utilize this ELISA to quantify 5HTR1A levels, providing insights into receptor expression and potential therapeutic applications.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-100%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.