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Quantitative Human 5HTR1A (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX209)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human 5HTR1A (glycosylated) sandwich ELISA kit is designed to detect human 5-hydroxytryptamine receptor 1A (5HTR1A) levels. 5HTR1A is a G protein-coupled receptor that binds serotonin, playing a crucial role in regulating mood, anxiety, and various physiological processes in the central nervous system. It is a target for numerous antidepressant and anxiolytic drugs. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.092 ng/mL, which can accurately detect low concentrations of 5HTR1A in the sample.
Target 5HTR1A
N-Glycosylation Site 10, 11, 24
Sample Types Tissue homogenates
Sample Volume 100 μL
Sensitivity 0.092 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name 5-Hydroxytryptamine receptor 1A
Alternate Names 5HTR1A; 5-Hydroxytryptamine receptor 1A; 5HTR1-A; Serotonin receptor 1A
Uniprot No. P08908
Application The quantitative human 5HTR1A (glycosylated) sandwich ELISA kit is valuable in research investigating psychiatric disorders such as depression and anxiety, as well as studies on the effects of serotonergic drugs. Researchers utilize this ELISA to quantify 5HTR1A levels, providing insights into receptor expression and potential therapeutic applications.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-100%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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