Quantitative Human AGRN (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX15)

Product Description	The quantitative human AGRN (glycosylated) sandwich ELISA kit is designed to detect human agrin (AGRN) levels. AGRN is a large extracellular matrix proteoglycan that plays a critical role in neuromuscular junction formation and maintenance. It is essential for synaptic differentiation and the organization of acetylcholine receptors at the postsynaptic membrane. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 14.974 pg/mL, which can accurately detect low concentrations of AGRN in the sample.
Target	AGRN
N-Glycosylation Site	135, 250, 777, 932
O-Glycosylation Site	1835
Sample Types	Tissue homogenates, serum, plasma
Sample Volume	100 μL
Sensitivity	14.974 pg/mL
Detection Principle	Quantitative sandwich ELISA
Detection Range	50 pg/mL-2000 pg/mL
Detection Time	1 h-5 h
Detection Wavelength	450 nm
Storage	Store at 2-8°C for long term storage.

Species	Human
Full Name	Agrin
Alternate Names	AGRN; Agrin; Agrin proteoglycan
Uniprot No.	O00468

Application	The quantitative human AGRN (glycosylated) sandwich ELISA kit is valuable for researchers investigating neuromuscular disorders, such as congenital myasthenic syndromes and amyotrophic lateral sclerosis, where AGRN function is often disrupted. By quantifying AGRN levels, researchers can gain insights into the role of this protein in disease pathogenesis and explore potential therapeutic interventions.
Kit Components	Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision	Intra-Assay: n=20, CV <8%; Inter-Assay: n=20, CV <10%;
Recovery	Serum sample: n=5, 85-100%; Plasma sample: n=4, 80-100%;
Standard Curve	The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.