Quantitative Human AGRN (Glycosylated) Sandwich ELISA Kit

The quantitative human AGRN (glycosylated) sandwich ELISA kit is designed to detect human agrin (AGRN) levels. AGRN is a large extracellular matrix proteoglycan that plays a critical role in neuromuscular junction formation and maintenance. It is essential for synaptic differentiation and the organization of acetylcholine receptors at the postsynaptic membrane. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 14.974 pg/mL, which can accurately detect low concentrations of AGRN in the sample.

AGRN

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, serum, plasma

100 μL

14.974 pg/mL

Quantitative sandwich ELISA

50 pg/mL-2000 pg/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Agrin

AGRN; Agrin; Agrin proteoglycan

135, 250, 777, 932

1835

The quantitative human AGRN (glycosylated) sandwich ELISA kit is valuable for researchers investigating neuromuscular disorders, such as congenital myasthenic syndromes and amyotrophic lateral sclerosis, where AGRN function is often disrupted. By quantifying AGRN levels, researchers can gain insights into the role of this protein in disease pathogenesis and explore potential therapeutic interventions.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-100%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.