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Quantitative Human APOB (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX164)

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1×48 T
1×96 T

Product Description The quantitative human APOB (glycosylated) sandwich ELISA kit is designed to detect human apolipoprotein B (APOB) levels. APOB is a major protein component of lipoproteins, playing a crucial role in lipid transport and metabolism. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.102 µg/mL, which can accurately detect low concentrations of APOB in the sample.
Target APOB
N-Glycosylation Site 34, 185, 983, 1368, 1377, 1523, 2239, 2560, 2779, 2982, 3101, 3224, 3336, 3358, 3411, 3465, 3895, 4237, 4431
Sample Types Tissue homogenates, cell lysates, serum, plasma
Sample Volume 100 μL
Sensitivity 0.102 µg/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 µg/mL-10 µg/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Apolipoprotein B
Alternate Names APOB; Apolipoprotein B; FLDB
Uniprot No. P04114
Application The quantitative human APOB (glycosylated) sandwich ELISA kit is valuable in research and clinical settings for assessing cardiovascular risk, monitoring lipid metabolism disorders, and evaluating the efficacy of lipid-lowering therapies. Researchers and clinicians utilize this ELISA to quantify APOB levels, providing insights into lipoprotein profiles and potential therapeutic interventions.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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