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Quantitative Human AQP2 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX405

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Specifications
Product Description
The quantitative human AQP2 (glycosylated) sandwich ELISA kit is designed to detect human aquaporin 2 (AQP2) levels. AQP2 is a water channel protein primarily found in the kidneys, which plays a critical role in regulating water reabsorption in the collecting ducts. The kit is suitable for various biological samples such as tissue homogenates, urine. Its sensitivity is 0.142 ng/mL, which can accurately detect low concentrations of AQP2 in the sample.
Target
AQP2
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, urine
Sample Volume
100 μL
Sensitivity
0.142 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.55 ng/mL-15 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Aquaporin 2
Alternate Names
AQP2; Aquaporin 2; AQP-CD; Aquaporin-CD; Collecting duct water channel protein
Uniprot No.
N-Glycosylation Site
123
Application Notes
Application
The quantitative human AQP2 (glycosylated) sandwich ELISA kit is used to measure glycosylated AQP2 levels in samples. This measurement is valuable in research and clinical settings for understanding kidney functions and water balance disorders. The kit is applied in studies of conditions such as diabetes insipidus, syndrome of inappropriate antidiuretic hormone secretion (SIADH), and other renal diseases.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-100%;
Plasma sample: n=4, 85-105%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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