Quantitative Human C5A (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX133)

Name: Quantitative Human C5A (Glycosylated) Sandwich ELISA Kit
SKU: GLJF-0525-HX133
Rating: 5 (1 reviews)

Datasheet

Size	Qty	Add To Basket
1×48 T
1×96 T

Product Description	The quantitative human C5A (glycosylated) sandwich ELISA kit is designed to detect human complement component 5a (C5A) levels. C5A is a potent anaphylatoxin generated from the cleavage of complement component 5 (C5), playing a key role in inflammation and immune response through its chemotactic and activating effects on various immune cells. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 30.027 pg/mL, which can accurately detect low concentrations of C5A in the sample.
Target	C5A
N-Glycosylation Site	741, 911, 1115, 1630
Sample Types	Tissue homogenates, cell lysates, serum, plasma
Sample Volume	100 μL
Sensitivity	30.027 pg/mL
Detection Principle	Quantitative sandwich ELISA
Detection Range	100 pg/mL-4500 pg/mL
Detection Time	1 h-5 h
Detection Wavelength	450 nm
Storage	Store at 2-8°C for long term storage.

Application	The quantitative human C5A (glycosylated) sandwich ELISA kit is valuable in research exploring the role of C5A in inflammatory diseases, sepsis, and autoimmune conditions. Researchers employ this ELISA to quantify C5A levels, providing insights into its involvement in pathological processes and potential therapeutic interventions targeting C5A-mediated inflammation.
Kit Components	Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision	Intra-Assay: n=20, CV <8%; Inter-Assay: n=20, CV <10%;
Recovery	Serum sample: n=5, 80-100%; Plasma sample: n=4, 80-100%;
Standard Curve	The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.

For Research Use Only.

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