Quantitative Human C5A (Glycosylated) Sandwich ELISA Kit

The quantitative human C5A (glycosylated) sandwich ELISA kit is designed to detect human complement component 5a (C5A) levels. C5A is a potent anaphylatoxin generated from the cleavage of complement component 5 (C5), playing a key role in inflammation and immune response through its chemotactic and activating effects on various immune cells. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 30.027 pg/mL, which can accurately detect low concentrations of C5A in the sample.

C5A

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates, serum, plasma

100 μL

30.027 pg/mL

Quantitative sandwich ELISA

100 pg/mL-4500 pg/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Complement component 5A

C5A; Complement component 5A

741, 911, 1115, 1630

The quantitative human C5A (glycosylated) sandwich ELISA kit is valuable in research exploring the role of C5A in inflammatory diseases, sepsis, and autoimmune conditions. Researchers employ this ELISA to quantify C5A levels, providing insights into its involvement in pathological processes and potential therapeutic interventions targeting C5A-mediated inflammation.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 80-100%;
Plasma sample: n=4, 80-100%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.