Quantitative Human CD8B (Glycosylated) Sandwich ELISA Kit

The quantitative human CD8B (glycosylated) sandwich ELISA kit is designed to detect human cluster of differentiation 8B (CD8B) levels. CD8B is a subunit of the CD8 co-receptor, expressed on cytotoxic T cells, playing a crucial role in antigen recognition and T cell activation. It cooperates with CD8A to form the functional CD8 heterodimer. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.882 ng/mL, which can accurately detect low concentrations of CD8B in the sample.

CD8B

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates

100 μL

0.882 ng/mL

Quantitative sandwich ELISA

2.50 ng/mL-100 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Cluster of differentiation 8B

CD8B; Cluster of differentiation 8B; CD8B1; CD8 antigen, Beta Polypeptide 1

102

The quantitative human CD8B (glycosylated) sandwich ELISA kit is valuable in research investigating immune responses, particularly cytotoxic T cell activity in cancer, viral infections, and autoimmune diseases. Researchers utilize this ELISA to quantify CD8B levels, providing insights into T cell populations and potential immunotherapeutic applications.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 80-95%;
Plasma sample: n=4, 85-100%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.