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Quantitative Human CD8B (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX223)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human CD8B (glycosylated) sandwich ELISA kit is designed to detect human cluster of differentiation 8B (CD8B) levels. CD8B is a subunit of the CD8 co-receptor, expressed on cytotoxic T cells, playing a crucial role in antigen recognition and T cell activation. It cooperates with CD8A to form the functional CD8 heterodimer. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.882 ng/mL, which can accurately detect low concentrations of CD8B in the sample.
Target CD8B
N-Glycosylation Site 102
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.882 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 2.50 ng/mL-100 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Cluster of differentiation 8B
Alternate Names CD8B; Cluster of differentiation 8B; CD8B1; CD8 antigen, Beta Polypeptide 1
Uniprot No. P10966
Application The quantitative human CD8B (glycosylated) sandwich ELISA kit is valuable in research investigating immune responses, particularly cytotoxic T cell activity in cancer, viral infections, and autoimmune diseases. Researchers utilize this ELISA to quantify CD8B levels, providing insights into T cell populations and potential immunotherapeutic applications.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-95%;
Plasma sample: n=4, 85-100%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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