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Quantitative Human CD97 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX434

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Specifications
Product Description
The quantitative human CD97 (glycosylated) sandwich ELISA kit is designed to detect human CD97 antigen (CD97) levels. CD97 is a cell surface protein belonging to the epidermal growth factor-like seven-transmembrane domain family. It is expressed on various cell types, including leukocytes and tumor cells. CD97 is involved in cell adhesion and migration. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.094 ng/mL, which can accurately detect low concentrations of CD97 in the sample.
Target
CD97
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates, serum, plasma
Sample Volume
100 μL
Sensitivity
0.094 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
CD97 antigen
Alternate Names
CD97; CD97 antigen; CD97 molecule
Uniprot No.
N-Glycosylation Site
33, 38, 108, 203, 371, 406, 413, 453, 520
Application Notes
Application
The quantitative human CD97 (glycosylated) sandwich ELISA kit is designed to measure glycosylated CD97 levels in samples. This measurement is valuable in research related to immunology and cancer biology. The kit is applied in studies of conditions such as inflammation and tumor metastasis, which helps in understanding the role of CD97.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 85-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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