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Quantitative Human CLCA1 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX5

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Specifications
Product Description
The quantitative human CLCA1 (glycosylated) sandwich ELISA kit is designed to detect human chloride channel accessory 1 (CLCA1) levels. CLCA1 is a protein encoded by the CLCA1 gene, and it belongs to the calcium-sensitive chloride conductance protein family. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.125 ng/mL, which can accurately detect low concentrations of CLCA1 in the sample.
Target
CLCA1
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.125 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Chloride channel accessory 1
Alternate Names
CLCA1; Chloride channel accessory 1; CLCRG1; Calcium-activated chloride channel protein 1
Uniprot No.
N-Glycosylation Site
503, 585, 770, 804, 810, 831, 836, 890
Application Notes
Application
The quantitative human CLCA1 (glycosylated) sandwich ELISA kit is designed for the quantitative determination of glycosylated CLCA1 concentrations in biological fluids. This kit utilizes a sandwich enzyme-linked immunosorbent assay technique, allowing researchers to measure CLCA1 levels for various research applications, including studies on inflammation, mucus production, and respiratory diseases.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-100%;
Plasma sample: n=4, 85-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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