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Quantitative Human CTSL (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX191

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Specifications
Product Description
The quantitative human CTSL (glycosylated) sandwich ELISA kit is designed to detect human cathepsin L (CTSL) levels. CTSL is a lysosomal cysteine protease involved in protein degradation, playing a role in various cellular processes, including antigen presentation, bone remodeling, and cancer progression. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.092 ng/mL, which can accurately detect low concentrations of CTSL in the sample.
Target
CTSL
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates, serum, plasma
Sample Volume
100 μL
Sensitivity
0.092 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Cathepsin L
Alternate Names
CTSL; Cathepsin L; CTSL1; MEP; Cathepsin L1; Major excreted protein
Uniprot No.
N-Glycosylation Site
221
Application Notes
Application
The quantitative human CTSL (glycosylated) sandwich ELISA kit is valuable in research investigating cancer, arthritis, and other diseases where CTSL is implicated in tissue remodeling and degradation. Researchers utilize this ELISA to quantify CTSL levels, providing insights into its role in disease pathogenesis and potential therapeutic targeting.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-100%;
Plasma sample: n=4, 85-105%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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