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Quantitative Human CTSL (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX191)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human CTSL (glycosylated) sandwich ELISA kit is designed to detect human cathepsin L (CTSL) levels. CTSL is a lysosomal cysteine protease involved in protein degradation, playing a role in various cellular processes, including antigen presentation, bone remodeling, and cancer progression. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.092 ng/mL, which can accurately detect low concentrations of CTSL in the sample.
Target CTSL
N-Glycosylation Site 221
Sample Types Tissue homogenates, cell lysates, serum, plasma
Sample Volume 100 μL
Sensitivity 0.092 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Cathepsin L
Alternate Names CTSL; Cathepsin L; CTSL1; MEP; Cathepsin L1; Major excreted protein
Uniprot No. P07711
Application The quantitative human CTSL (glycosylated) sandwich ELISA kit is valuable in research investigating cancer, arthritis, and other diseases where CTSL is implicated in tissue remodeling and degradation. Researchers utilize this ELISA to quantify CTSL levels, providing insights into its role in disease pathogenesis and potential therapeutic targeting.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-100%;
Plasma sample: n=4, 85-105%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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