Quantitative Human DCD (Glycosylated) Sandwich ELISA Kit

The quantitative human DCD (glycosylated) sandwich ELISA kit is designed to detect human dermcidin (DCD) levels. DCD is a small, antimicrobial peptide that is primarily found in human sweat. DCD plays a crucial role in the skin's defense against microbial infections, which exhibits broad-spectrum antimicrobial activity against various bacteria, fungi, and yeasts. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.525 ng/mL, which can accurately detect low concentrations of DCD in the sample.

DCD

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates

100 μL

0.525 ng/mL

Quantitative sandwich ELISA

2.50 ng/mL-50 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Dermcidin

DCD; Dermcidin; HCAP; DCD1; PIF; Proteolysis inducing factor; Preproteolysin

30, 38

The quantitative human DCD (glycosylated) sandwich ELISA kit provides a method for the quantitative determination of glycosylated DCD levels in biological samples. Researchers use it to investigate the role of DCD in skin health, wound healing, and defense against infection. The kit has applications in studies of skin biology, infectious diseases, and the development of novel antimicrobial therapies.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 80-95%;
Plasma sample: n=4, 90-105%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.