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Quantitative Human DMT1 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX436

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Specifications
Product Description
The quantitative human DMT1 (glycosylated) sandwich ELISA kit is designed to detect human divalent metal transporter 1 (DMT1) levels. DMT1 is a protein responsible for transporting iron and other divalent metals across cell membranes. It is crucial for iron absorption in the intestine and iron transport in other tissues. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.079 ng/mL, which can accurately detect low concentrations of DMT1 in the sample.
Target
DMT1
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.079 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Divalent metal transporter 1
Alternate Names
DMT1; Divalent metal transporter 1; SLC11A2; NRAMP2; Natural resistance-associated macrophage protein 2
Uniprot No.
N-Glycosylation Site
336, 349
Application Notes
Application
The quantitative human DMT1 (glycosylated) sandwich ELISA kit is designed to measure glycosylated DMT1 levels in samples. This measurement is valuable in research related to iron metabolism and related disorders. The kit is applied in studies of conditions such as anemia, which helps in understanding the role of DMT1.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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