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Quantitative Human DNM1L (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX14

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Specifications
Product Description
The quantitative human DNM1L (glycosylated) sandwich ELISA kit is designed to detect human dynamin 1 like protein (DNM1L) levels. DNM1L is a GTPase involved in mitochondrial fission, a process essential for mitochondrial dynamics and cellular function. It plays a critical role in regulating mitochondrial morphology and distribution. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.082 ng/mL, which can accurately detect low concentrations of DNM1L in the sample.
Target
DNM1L
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.082 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Dynamin 1 like protein
Alternate Names
DNM1L; Dynamin 1 like protein; DRP1; DLP1; Dynamin-related protein 1
Uniprot No.
O-Glycosylation Site
585, 586
Application Notes
Application
The quantitative human DNM1L (glycosylated) sandwich ELISA kit is valuable for researchers investigating mitochondrial dysfunction in neurodegenerative diseases, cancer, and metabolic disorders, where DNM1L activity is often altered. By quantifying DNM1L, researchers can gain insights into the role of mitochondrial dynamics in disease pathogenesis and explore potential therapeutic targets.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 90-110%;
Plasma sample: n=4, 85-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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