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Quantitative Human EAAT2 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX417)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human EAAT2 (glycosylated) sandwich ELISA kit is designed to detect human excitatory amino acid transporter 2 (EAAT2) levels. EAAT2 is a glutamate transporter protein located in the brain, which plays a critical role in removing glutamate from the synaptic cleft. EAAT2 is essential for regulating glutamate neurotransmission. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.120 ng/mL, which can accurately detect low concentrations of EAAT2 in the sample.
Target EAAT2
N-Glycosylation Site 206, 216
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.120 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 0.55 ng/mL-15 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Excitatory amino acid transporter 2
Alternate Names EAAT2; Excitatory amino acid transporter 2; SLC1A2; GLT-1; Solute carrier family 1 member 2
Uniprot No. P43004
Application The quantitative human EAAT2 (glycosylated) sandwich ELISA kit is used to measure glycosylated EAAT2 levels in samples. This measurement is important in neuroscience research, especially for studying glutamate signaling and neurological disorders. The kit is applied in investigations of conditions such as Alzheimer's disease and epilepsy.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 85-105%;
Plasma sample: n=4, 90-110%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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