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Quantitative Human EAAT3 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX418)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human EAAT3 (glycosylated) sandwich ELISA kit is designed to detect human excitatory amino acid transporter 3 (EAAT3) levels. EAAT3 is another glutamate transporter protein found in neurons. Like EAAT2, it removes glutamate from the synaptic cleft, regulating neurotransmission. EAAT3 contributes to the overall control of glutamate levels in the brain. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.125 ng/mL, which can accurately detect low concentrations of EAAT3 in the sample.
Target EAAT3
N-Glycosylation Site 43, 178, 195
Sample Types Tissue homogenates
Sample Volume 100 μL
Sensitivity 0.125 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 0.55 ng/mL-15 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Excitatory amino acid transporter 3
Alternate Names EAAT3; Excitatory amino acid transporter 3; SLC1A1; Solute carrier 1 member 1
Uniprot No. P43005
Application The quantitative human EAAT3 (glycosylated) sandwich ELISA kit is designed to measure glycosylated EAAT3 levels in samples. This measurement is valuable in neuroscience research, particularly for studying glutamate signaling and neurological diseases. The kit is applied in investigations of conditions where glutamate dysregulation is implicated.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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