Quantitative Human EAAT3 (Glycosylated) Sandwich ELISA Kit

The quantitative human EAAT3 (glycosylated) sandwich ELISA kit is designed to detect human excitatory amino acid transporter 3 (EAAT3) levels. EAAT3 is another glutamate transporter protein found in neurons. Like EAAT2, it removes glutamate from the synaptic cleft, regulating neurotransmission. EAAT3 contributes to the overall control of glutamate levels in the brain. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.125 ng/mL, which can accurately detect low concentrations of EAAT3 in the sample.

EAAT3

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates

100 μL

0.125 ng/mL

Quantitative sandwich ELISA

0.55 ng/mL-15 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Excitatory amino acid transporter 3

EAAT3; Excitatory amino acid transporter 3; SLC1A1; Solute carrier 1 member 1

43, 178, 195

The quantitative human EAAT3 (glycosylated) sandwich ELISA kit is designed to measure glycosylated EAAT3 levels in samples. This measurement is valuable in neuroscience research, particularly for studying glutamate signaling and neurological diseases. The kit is applied in investigations of conditions where glutamate dysregulation is implicated.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-95%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.