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Quantitative Human ERLIN1 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX89

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Specifications
Product Description
The quantitative human ERLIN1 (glycosylated) sandwich ELISA kit is designed to detect human endoplasmic reticulum lipid raft associated protein 1 (ERLIN1) levels. ERLIN1 is a protein involved in ER-associated degradation (ERAD) and lipid raft organization, playing roles in cellular homeostasis and signaling. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.181 ng/mL, which can accurately detect low concentrations of ERLIN1 in the sample.
Target
ERLIN1
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates
Sample Volume
100 μL
Sensitivity
0.181 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.55 ng/mL-15 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Endoplasmic reticulum lipid raft associated protein 1
Alternate Names
ERLIN1; Endoplasmic reticulum lipid raft associated protein 1; C10orf69; Erlin-1; SPFH1
Uniprot No.
N-Glycosylation Site
108
Application Notes
Application
The quantitative human ERLIN1 (glycosylated) sandwich ELISA kit is valuable for research investigating ERAD pathways, lipid raft dynamics, and diseases related to protein misfolding, such as neurodegenerative disorders. The quantitative data obtained from this kit allows for precise analysis of ERLIN1 levels, contributing to a better understanding of its function in cellular processes and disease pathogenesis.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 85-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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