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Quantitative Human ERN1 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX87)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human ERN1 (glycosylated) sandwich ELISA kit is designed to detect human endoplasmic reticulum to nucleus signalling 1 (ERN1) levels. ERN1 is a transmembrane protein that acts as a sensor of endoplasmic reticulum (ER) stress and initiates the unfolded protein response (UPR). The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.091 ng/mL, which can accurately detect low concentrations of ERN1 in the sample.
Target ERN1
N-Glycosylation Site 176
Sample Types Tissue homogenates, serum, plasma
Sample Volume 100 μL
Sensitivity 0.091 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Endoplasmic reticulum to nucleus signalling 1
Alternate Names ERN1; Endoplasmic reticulum to nucleus signalling 1; IRE1; Inositol-requiring protein 1
Uniprot No. O75460
Application The quantitative human ERN1 (glycosylated) sandwich ELISA kit is valuable for research investigating ER stress-related diseases, such as neurodegenerative disorders, diabetes, and cancer. The quantitative data obtained from this kit allows for precise analysis of ERN1 levels, contributing to a better understanding of its role in cellular stress responses and disease pathogenesis.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 85-105%;
Plasma sample: n=4, 80-100%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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