Quantitative Human ERN1 (Glycosylated) Sandwich ELISA Kit

The quantitative human ERN1 (glycosylated) sandwich ELISA kit is designed to detect human endoplasmic reticulum to nucleus signalling 1 (ERN1) levels. ERN1 is a transmembrane protein that acts as a sensor of endoplasmic reticulum (ER) stress and initiates the unfolded protein response (UPR). The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.091 ng/mL, which can accurately detect low concentrations of ERN1 in the sample.

ERN1

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, serum, plasma

100 μL

0.091 ng/mL

Quantitative sandwich ELISA

1 ng/mL-10 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Endoplasmic reticulum to nucleus signalling 1

ERN1; Endoplasmic reticulum to nucleus signalling 1; IRE1; Inositol-requiring protein 1

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The quantitative human ERN1 (glycosylated) sandwich ELISA kit is valuable for research investigating ER stress-related diseases, such as neurodegenerative disorders, diabetes, and cancer. The quantitative data obtained from this kit allows for precise analysis of ERN1 levels, contributing to a better understanding of its role in cellular stress responses and disease pathogenesis.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 85-105%;
Plasma sample: n=4, 80-100%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.