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Quantitative Human IgE (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX139)

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1×48 T
1×96 T

Product Description The quantitative human IgE (glycosylated) sandwich ELISA kit is designed to detect human immunoglobulin E (IgE) levels. IgE is an antibody isotype primarily associated with allergic reactions and defense against parasitic infections. It triggers mast cell and basophil degranulation, leading to the release of inflammatory mediators. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.801 µg/mL, which can accurately detect low concentrations of IgE in the sample.
Target IgE
N-Glycosylation Site 21, 49, 99, 146, 252, 264, 275
Sample Types Tissue homogenates, cell lysates, serum, plasma
Sample Volume 100 μL
Sensitivity 0.801 µg/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 2 µg/mL-100 µg/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Immunoglobulin E
Alternate Names IgE; Immunoglobulin E; IGHE; Immunoglobulin heavy constant epsilon
Uniprot No. P01854
Application The quantitative human IgE (glycosylated) sandwich ELISA kit is crucial for diagnosing and monitoring allergic diseases, as well as investigating IgE-mediated immune responses. Researchers and clinicians use this ELISA to quantify IgE levels, aiding in the identification of allergens and the assessment of disease severity.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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