Quantitative Human IgE (Glycosylated) Sandwich ELISA Kit

The quantitative human IgE (glycosylated) sandwich ELISA kit is designed to detect human immunoglobulin E (IgE) levels. IgE is an antibody isotype primarily associated with allergic reactions and defense against parasitic infections. It triggers mast cell and basophil degranulation, leading to the release of inflammatory mediators. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.801 µg/mL, which can accurately detect low concentrations of IgE in the sample.

IgE

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates, serum, plasma

100 μL

0.801 µg/mL

Quantitative sandwich ELISA

2 µg/mL-100 µg/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Immunoglobulin E

IgE; Immunoglobulin E; IGHE; Immunoglobulin heavy constant epsilon

21, 49, 99, 146, 252, 264, 275

The quantitative human IgE (glycosylated) sandwich ELISA kit is crucial for diagnosing and monitoring allergic diseases, as well as investigating IgE-mediated immune responses. Researchers and clinicians use this ELISA to quantify IgE levels, aiding in the identification of allergens and the assessment of disease severity.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-95%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.