Quantitative Human KMO (Glycosylated) Sandwich ELISA Kit

The quantitative human KMO (glycosylated) sandwich ELISA kit is designed to detect human kynurenine-3-monooxygenase (KMO) levels. KMO is an enzyme that catalyzes the hydroxylation of kynurenine to 3-hydroxykynurenine, a key step in the kynurenine pathway of tryptophan metabolism. It plays a role in the production of neuroactive metabolites and is implicated in various neurological and inflammatory conditions. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.513 ng/mL, which can accurately detect low concentrations of KMO in the sample.

KMO

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates

100 μL

0.513 ng/mL

Quantitative sandwich ELISA

2 ng/mL-50 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Kynurenine-3-monooxygenase

KMO; Kynurenine-3-monooxygenase; Kynurenine 3-hydroxylase

465

The quantitative human KMO (glycosylated) sandwich ELISA kit is valuable for researchers investigating neurodegenerative diseases, psychiatric disorders, and inflammatory diseases, where KMO activity is often altered. By quantifying KMO, researchers can gain insights into the role of the kynurenine pathway in disease pathogenesis and explore potential therapeutic targets related to tryptophan metabolism.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 80-100%;
Plasma sample: n=4, 85-105%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.