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Quantitative Human LIFR (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX414

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Specifications
Product Description
The quantitative human LIFR (glycosylated) sandwich ELISA kit is designed to detect human leukemia inhibitory factor receptor (LIFR) levels. LIFR is a receptor for the cytokine leukemia inhibitory factor (LIF). LIF is involved in various biological processes, including cell growth, differentiation, and inflammation. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.128 ng/mL, which can accurately detect low concentrations of LIFR in the sample.
Target
LIFR
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates, serum, plasma
Sample Volume
100 μL
Sensitivity
0.128 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.55 ng/mL-15 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Leukemia inhibitory factor receptor
Alternate Names
LIFR; Leukemia inhibitory factor receptor; CD118
Uniprot No.
N-Glycosylation Site
64, 85, 131, 143, 191, 243, 303, 390, 407, 426, 445, 481, 489, 572, 652, 663, 680, 729, 787
Application Notes
Application
The quantitative human LIFR (glycosylated) sandwich ELISA kit is designed to measure glycosylated LIFR levels in samples. This measurement is valuable in research related to cell signaling, development, and inflammation. The kit is applied in studies of conditions such as cancer, inflammatory diseases, and reproductive disorders.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-100%;
Plasma sample: n=4, 85-110%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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